Histological evaluation of direct pulp capping of rat pulp with experimentally developed low-viscosity adhesives containing reparative dentin-promoting agents
| Autor: | Masaya Suzuki, Koichi Shinkai, Yoshiroh Katoh, Chikage Kato, Yoshihisa Taira |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Materials science chemistry.chemical_element Calcium Dentin Secondary Dental Pulp Capping Rats Sprague-Dawley Calcium Chloride 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine stomatognathic system Adhesives Dentin medicine Animals Dental Pulp Exposure Composite material Dental Restoration Permanent General Dentistry Dental Pulp Fumed silica Reparative dentin Calcium hydroxide Pulpitis 030206 dentistry Rats Resin Cements Root Canal Therapy Pulp capping 030104 developmental biology medicine.anatomical_structure chemistry Dentin-Bonding Agents Pulp (tooth) Adhesive Pulp Capping and Pulpectomy Agents Nuclear chemistry |
| Zdroj: | Journal of Dentistry. 44:27-36 |
| ISSN: | 0300-5712 |
| DOI: | 10.1016/j.jdent.2015.11.005 |
| Popis: | Objectives This study examines the wound healing process in exposed rat pulp when capped with experimental adhesive resin systems. Methods Experimental adhesive resin system for direct pulp capping was composed of primer-I (PI), -II (PII), and -III (PIII) and an experimental bonding agent (EBA). PI was Clearfil ® SE Bond ® /Primer (CSP) containing 5.0 wt% CaCl 2 , PII was PI containing 10 wt% nanofiller (Aerosil ® 380), and PIII was CSP containing 5.0 wt% of compounds of equal moles of synthetic peptides (pA and pB) derived from dentin matrix protein 1. EBA was Clearfil ® SE Bond ® /Bond (CSB) containing 10 wt% hydroxyapatite powders. Three experimental groups were designed. PI was assigned to experimental Groups 1 and 3. PII was assigned to experimental Groups 2 and 3. PIII and EBA were assigned to all experimental adhesive groups. Control teeth were capped with calcium hydroxide preparation (Dycal ® ), and CSP and CSB were applied to the cavity. The rats were sacrificed after each observation period (14, 28, 56, and 112 days). The following parameters were evaluated: pulp tissue disorganization, inflammatory cell infiltration, reparative dentin formation (RDF), and bacterial penetration. Results There were no significant differences among all the groups for all parameters and all observation periods ( p > 0.05, Kruskal–Wallis test). All groups showed initial RDF at 14 days postoperatively and extensive RDF until 112 days postoperatively. Groups 2 and 3 demonstrated higher quantity of mineralized dentin bridge formation compared with Group 1. Conclusions Addition of nanofillers to the primer was effective in promoting high-density RDF. Clinical significance Experimentally developed adhesive resin systems induce the exposed pulp to produce almost the same quantity of reparative dentin as calcium hydroxide. However, we need further studies to elucidate whether the same results could be obtained in humans. |
| Databáze: | OpenAIRE |
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