Several subspecies and sequence types are associated with the emergence of Xylella fastidiosa in natural settings in France

Autor: Nicolas Denancé, Valérie Olivier, Bruno Legendre, Martial Briand, Françoise Poliakoff, Marie-Agnès Jacques, C. de Boisseson
Přispěvatelé: Institut de Recherche en Horticulture et Semences (IRHS), AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA)-Université d'Angers (UA), Laboratoire de la santé des végétaux, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Viral Genetics and Biosecurity, N.D. was funded by regional funds provided by Objectif Végétal. The work carried out at INRA has received funding from the European Union's Horizon 2020 research and innovation program under grant agreement 635646, POnTE (Pest Organisms Threatening Europe)., Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0106 biological sciences
0301 basic medicine
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
Phytopathology and phytopharmacy
Sequence analysis
Xylella fastidiosa subsp. pauca
030106 microbiology
coffee
Leaf scorch
multilocus sequence typing
Biotechnologies
Plant Science
comparative genomics
Horticulture
Subspecies
01 natural sciences
Polygala myrtifolia
03 medical and health sciences
Botany
gène recombinant
Genetics
medicine
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Typing
comparaison génomique
Comparative genomics
Vegetal Biology
biology
natural selection
biology.organism_classification
medicine.disease
Phytopathologie et phytopharmacie
bactérie de quarantaine
[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
Xylella fastidiosa subsp. multiplex
Multilocus sequence typing
Xylella fastidiosa
sélection naturelle
Agronomy and Crop Science
Biologie végétale
010606 plant biology & botany
Zdroj: Plant Pathology
Plant Pathology, Wiley, 2017, ⟨10.1111/ppa.12695⟩
Plant Pathology (66), 1054-1064. (2017)
Plant Pathology, Wiley, 2017, 66, ⟨10.1111/ppa.12695⟩
ISSN: 0032-0862
1365-3059
Popis: Xylella fastidiosa is a plant pathogenic bacterium emerging in Europe. In France its emergence has been demonstrated through interceptions of contaminated coffee plants and, in 2015, by a survey of natural settings. The first French focus of contamination was detected in 2015 in Corsica; since then, almost 300 foci have been found and nearly 30 plant species have been declared contaminated, with Polygala myrtifolia remaining the principal host, suffering from severe leaf scorch. This study reports on the diversity of X. fastidiosa identified in France in 2015. Multilocus sequence analysis/typing revealed the presence of mainly X. fastidiosa subsp. multiplex sequence types (STs) ST6 and ST7. A focus of X. fastidiosa subsp. pauca ST53 was identified in mainland France; one sample contaminated by X. fastidiosa subsp. sandyi ST76, one novel recombinant, and coinfections of different isolates in individual samples were also identified, but could not be confirmed by successive samplings, indicating limited or transient contamination. Koch's postulates were fulfilled for two isolates of X. fastidiosa subsp. multiplex on P. myrtifolia, one being ST6 and the other ST7. Comparative genomics of the genome sequences of three French isolates (one ST6 and two ST7) with available sequences revealed that, unlike the American Dixon strain, the French ST6 and ST7 strains are devoid of a plasmid encoding a complete type IV secretion system. Other differences regarding phage sequences were highlighted. Altogether, the results suggest that the emergence of X. fastidiosa in France is linked to several introduction events of diverse strains from different subspecies.
Databáze: OpenAIRE
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