A Rho GTPase controls the rate of protein synthesis in the sea urchin egg
| Autor: | Mario Pedraza-Reyes, Jesús García-Soto, Juana López-Godínez, Guadalupe Martínez-Cadena, Salvador Manzo |
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| Rok vydání: | 2003 |
| Předmět: |
rho GTP-Binding Proteins
Cytoplasm Botulinum Toxins Time Factors G protein Biophysics GTPase Microfilament Biochemistry Exocytosis biology.animal Animals Molecular Biology Sea urchin Cytoskeleton biology Cortical granule exocytosis Oocyte activation Cell Biology Cell biology Microscopy Fluorescence Fertilization Protein Biosynthesis Sea Urchins embryonic structures Calcium rhoA GTP-Binding Protein Cytokinesis Signal Transduction Subcellular Fractions |
| Zdroj: | Biochemical and Biophysical Research Communications. 310:685-690 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2003.08.153 |
| Popis: | Fertilization of the sea urchin egg triggers a Ca(2+)-dependent cortical granule exocytosis and cytoskeletal reorganization, both of which are accompanied by an accelerated protein synthesis. The signaling mechanisms leading to these events are not completely understood. The possible role of Rho GTPases in sea urchin egg activation was studied using the Clostridium botulinum C3 exotoxin, which specifically ADP-ribosylates Rho proteins and inactivates them. We observed that incubation of eggs with C3 resulted in in situ ADP-ribosylation of Rho. Following fertilization, C3-treated eggs were capable of performing cortical granule exocytosis but not the first cytokinesis. C3 caused in both unfertilized eggs and early embryos alterations in the state of actin polymerization and inhibition of the spindle formation. Moreover, C3 diminished markedly the rate of protein synthesis. These findings suggested that Rho is involved in regulating the acceleration of protein synthesis that accompanies the egg activation by sperm. |
| Databáze: | OpenAIRE |
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