Determination of the dietary lysine requirement by measuring plasma free lysine concentrations in rainbow trout Oncorhynchus mykiss after dorsal aorta cannulation
| Autor: | Kumar Katya, Hyeonho Yun, Imho Ok, Sungchul C. Bai, Gun-Jun Park, Silas S.O. Hung |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Lysine Aquatic Science Biology Oceanography Body weight Biochemistry complex mixtures lcsh:Aquaculture. Fisheries. Angling 03 medical and health sciences Plasma Animal science Casein Molecular Biology chemistry.chemical_classification lcsh:SH1-691 04 agricultural and veterinary sciences Amino acid 030104 developmental biology Dietary protein Rainbow trout chemistry Animal ecology Oncorhynchus mykiss 040102 fisheries 0401 agriculture forestry and fisheries Fish bacteria Dorsal aorta cannulation |
| Zdroj: | Fisheries and Aquatic Sciences, Vol 19, Iss 1, Pp 1-7 (2016) |
| ISSN: | 2234-1757 |
| Popis: | This study evaluated the dietary lysine requirement by measuring the plasma free lysine concentrations in rainbow trout, Oncorhynchus mykiss after dorsal aorta cannulation. A basal diet containing 36.6 % crude protein (29.6 % crystalline amino acids mixture, 5 % casein and 2 % gelatin) was formulated to one of the seven L-amino acid based diets containing graded levels of lysine (0.72, 1.12, 1.52, 1.92, 2.32, 2.72 or 3.52 % dry diet). A total of 35 fish averaging 512 ± 6.8 g (mean ± SD) were randomly distributed into seven groups with five fish in each group. After 48 h of feed deprivation, each group of fish was fed one of the experimental diets by intubation at 1 % body weight. Blood samples were taken at 0, 5 and 24 h after intubation. Post-prandial plasma free lysine concentrations (PPlys, 5 h after intubation) of fish fed diets containing ≥ 2.32 % lysine were higher than those of fish fed diets containing ≤ 1.92 % lysine. Post-absorptive free lysine concentrations (PAlys, 24 h after intubation) of fish fed diets containing 2.32 and 3.52 % lysine were higher than those of fish fed diets containing ≤ 1.52 % lysine. The broken-line regression analysis on the basis of PPlys and PAlys indicated that the lysine requirement of rainbow trout could be 2.34 and 2.20 % in diet. Therefore, these results strongly suggested that the dietary lysine requirement based on the broken-line model analyses of PPlys and PAlys could be greater than 2.2 but less than 2.34 % (corresponding to be 6.01 % ≤, but ≤ 6.39 % in dietary protein basis, respectively) in rainbow trout. Also, these results shown that the quantitative estimation of lysine requirement by using PPlys and PAlys could be an acceptable method in fish. |
| Databáze: | OpenAIRE |
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