PtoMYB170 positively regulates lignin deposition during wood formation in poplar and confers drought tolerance in transgenic Arabidopsis
| Autor: | Qiaoyan Tian, Bo Jiao, Keming Luo, Rui Liu, Xiaokang Fu, Li Guo, Lingyu Ran, Chaofeng Li, Bangjun Wang, Changzheng Xu |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Transgene Drought tolerance Arabidopsis Plant Science Genetically modified crops Lignin 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Plant Guard cell Botany Promoter Regions Genetic biology Gene Expression Profiling fungi food and beverages Xylem Plants Genetically Modified biology.organism_classification Wood Droughts Populus 030104 developmental biology chemistry Heterologous expression 010606 plant biology & botany |
| Zdroj: | Tree Physiology. 37:1713-1726 |
| ISSN: | 1758-4469 0829-318X |
| DOI: | 10.1093/treephys/tpx093 |
| Popis: | Wood formation is a complex developmental process under multi-level transcriptional control executed by a large set of transcription factors. However, only limited members have been characterized to be key regulators of lignin biosynthesis in poplar. Here we report the conserved and unique functions of PtoMYB170, a transcription factor identified from Populus tomentosa (Chinese white poplar), in lignin deposition and drought tolerance in comparison with its duplicate paralog PtoMYB216. PtoMYB170 is preferentially expressed in young leaves and xylem tissues. Overexpression of PtoMYB170 in transgenic poplar plants resulted in stronger lignification and more thickened secondary wall in xylem compared with wild-type plants, whereas the CRISPR/Cas9-generated mutation of PtoMYB170 weakened lignin deposition, thereby leading to a more flexible and collapsed xylem phenotype. Transient expression experiments demonstrated that PtoMYB170 specifically activated the expression of lignin biosynthetic genes, consistent with the function of PtoMYB216. However, GUS staining assays revealed that PtoMYB170 was specifically expressed in guard cells of transgenic Arabidopsis while PtoMYB216 was not. Heterologous expression of PtoMYB170 in Arabidopsis enhanced stomatal closure in the dark and resulted in drought tolerance of the transgenic plants through reduced water loss, indicating a diversified role from PtoMYB216. These results revealed the PtoMYB170-dependent positive transcriptional regulation on lignin deposition in poplar and its coordinated function in enhancing drought tolerance by promoting dark-induced stomatal closure. |
| Databáze: | OpenAIRE |
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