Anti-Müllerian hormone receptor type 2 (AMHR2) expression in bovine oviducts and endometria: comparison of AMHR2 mRNA and protein abundance between old Holstein and young and old Wagyu females
| Autor: | Onalenna Kereilwe, Hiroya Kadokawa, Raihana Nasrin Ferdousy |
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| Rok vydání: | 2019 |
| Předmět: |
Anti-Mullerian Hormone
endocrine system Aging Receptors Peptide Gene Expression Estrous Cycle Reproductive technology Biology Gonadotropic cell Endometrium Andrology Endocrinology Species Specificity Genetics medicine Animals RNA Messenger Receptor Molecular Biology Fallopian Tubes Messenger RNA urogenital system Antral follicle medicine.anatomical_structure Reproductive Medicine Immunohistochemistry Animal Science and Zoology Cattle Female Receptors Transforming Growth Factor beta Developmental Biology Biotechnology Hormone |
| Zdroj: | Reproduction, fertility, and development. 32(8) |
| ISSN: | 1031-3613 |
| Popis: | Anti-Müllerian hormone (AMH) is a glycoprotein produced by granulosa cells of preantral and small antral follicles that has multiple important roles in the ovaries. Recent studies have revealed extragonadal AMH regulation of gonadotrophin secretion from bovine gonadotrophs. In this study we investigated whether the primary receptor for AMH, AMH receptor type 2 (AMHR2), is expressed in bovine oviducts and endometria. Reverse transcription–polymerase chain reaction detected expression of AMHR2 mRNA in oviductal and endometrial specimens. Western blotting and immunohistochemistry were performed to analyse AMHR2 protein expression using anti-bovine AMHR2 antibody. Immunohistochemistry revealed robust AMHR2 expression in the tunica mucosa of the ampulla and isthmus, as well as in the glandular and luminal epithelium of the endometrium. AMHR2 mRNA (measured by real-time polymerase chain reaction) and AMHR2 protein expression in these layers did not significantly differ among oestrous phases in adult Wagyu cows (P>0.1). In addition, AMHR2 mRNA and protein expression in these layers did not differ among old Holsteins (mean (±s.e.m.) age 91.9±6.4 months) and young (26.6±0.8 months) and old (98.8±10.2 months) Wagyu cows. Therefore, AMHR2 is expressed in bovine oviducts and endometria. |
| Databáze: | OpenAIRE |
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