Ultrastructural morphology of goblet cells of the conjunctiva of dogs
| Autor: | Rosélia L. S Araújo, José R. Corrêa, Paula Diniz Galera |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
Pathology medicine.medical_specialty Conjunctiva 040301 veterinary sciences law.invention 0403 veterinary science 03 medical and health sciences 0302 clinical medicine Dogs Microscopy Electron Transmission law medicine Animals Keratoconjunctivitis General Veterinary Chemistry 04 agricultural and veterinary sciences medicine.disease Mucus eye diseases Staining medicine.anatomical_structure 030221 ophthalmology & optometry Ultrastructure Microscopy Electron Scanning Female sense organs Eyelid Goblet Cells Electron microscope Reticulum |
| Zdroj: | Veterinary ophthalmologyREFERENCES. 22(6) |
| ISSN: | 1463-5224 |
| Popis: | OBJECTIVE To describe the morphology of goblet cells of the eyelid conjunctiva in dogs using transmission and scanning electron microscopy. ANIMAL STUDIED Ten dogs, both male and female of different breeds, with no ocular changes were examined (20 eyes). PROCEDURES Ten samples of conjunctiva were collected and processed for scanning and transmission electron microscopy (SEM and TEM), while another 10 samples were stained with Schiff's periodic stain (SPA) and alcian blue, pH 2.5, and analyzed using light microscopy. RESULTS Scanning electron microscopy revealed several points of mucus extrusion in the free apical portion of the goblet cells as well as a wide distribution of lymphoid follicles and macrophages intermingling with the microvilli of palpebral epithelium cells. TEM revealed normal goblet cells that were predominantly oval with wide cytoplasm of different diameters, and large vesicles with heterogeneous granules and free edges, suggesting the release of mucus content onto the conjunctival surface. Cytoplasmic organelles, such as the Golgi apparatus, endoplasmic reticulum, and a high number of mitochondria were also observed. All the samples were positive for SPA and alcian blue staining. CONCLUSION This is the first study to evaluate the goblet cells of the eyelid conjunctiva in healthy dogs using electron microscopy techniques. These results are useful for comparing the palpebral conjunctiva of dogs without ocular changes to palpebral conjunctiva of dogs and other species with ocular changes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text