Differential effects of cyclo-oxygenase pathway metabolites on cytokine production by T lymphocytes
| Autor: | M Vanoli, M. Molteni, S. Compasso, S. Della Bella, Raffaella Scorza, C. Zulian |
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| Rok vydání: | 1997 |
| Předmět: |
Adult
medicine.medical_specialty Thromboxane T-Lymphocytes medicine.medical_treatment Clinical Biochemistry Prostacyclin Stimulation In Vitro Techniques Biology Dinoprostone Interferon-gamma Thromboxane A2 Adjuvants Immunologic Internal medicine medicine Humans Iloprost Platelet activation Prostaglandin E2 Arachidonic Acid Interleukin-6 Tumor Necrosis Factor-alpha Cell Biology Prostaglandin Endoperoxides Synthetic Kinetics Endocrinology Cytokine Eicosanoid Prostaglandin-Endoperoxide Synthases 15-Hydroxy-11 alpha 9 alpha-(epoxymethano)prosta-5 13-dienoic Acid Cytokines Interleukin-2 lipids (amino acids peptides and proteins) medicine.drug |
| Zdroj: | Prostaglandins, Leukotrienes and Essential Fatty Acids. 56:177-184 |
| ISSN: | 0952-3278 |
| DOI: | 10.1016/s0952-3278(97)90531-7 |
| Popis: | Cyclo-oxygenase pathway metabolites released in the microenvironment by activated platelets and endothelial cells are potential local modulators of the immune response. In the present study, we have investigated the modulatory role of PGE 2 , Iloprost (prostacyclin analogue), U-46619 (thromboxane analogue) on the release of IL-2, IFN-gamma, TNF-alpha and IL-6 by T lymphocytes. Our results show that PGE 2 and prostacyclin differ in the regulation of cytokine production. PGE 2 inhibited the release of IL-2 and IFN-gamma, while Iloprost did not affect their production. The addition of PGE 2 or Iloprost greatly decreased the amount of TNF-alpha measured in the supernatants, although the rates of inhibition differed according to the kind of stimulation. Unlike that of PGE 2 , inhibition by Iloprost was stronger in allocativated cultures than in PHA-stimulated ones. In vitro IL-6 production was stimulated by PGE 2 in alloactivated cultures and by Iloprost, whatever the stimulus. These results are probably due to other cellular subsets contaminating the T-lymphocyte preparations. After complete removal of monocytes from cell cultures, there were inhibitory effects of Iloprost and PGE 2 on IL-6 released in the supernatants. We did not observe any significant effect of thromboxane analogue on the production of either cytokine. |
| Databáze: | OpenAIRE |
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