Supramolecular Encapsulation of Small-Ultrared Fluorescent Proteins in Virus-Like Nanoparticles for Noninvasive In Vivo Imaging Agents
| Autor: | Michael A. Luzuriaga, Fabian C. Herbert, Tyler Galbraith, Olivia R. Brohlin, Jeremiah J. Gassensmith, Cesar Reyes, Arezoo Shahrivarkevishahi, Candace E. Benjamin |
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| Rok vydání: | 2020 |
| Předmět: |
viruses
Biomedical Engineering Supramolecular chemistry Pharmaceutical Science Nanoparticle Bioengineering Capsules 02 engineering and technology Fluorescent imaging 01 natural sciences Virus Green fluorescent protein Mice Phagocytosis Animals Pharmacology 010405 organic chemistry Chemistry Organic Chemistry biochemical phenomena metabolism and nutrition Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology Fluorescence 0104 chemical sciences Molecular Imaging Luminescent Proteins RAW 264.7 Cells Capsid Viruses Biophysics Nanoparticles 0210 nano-technology Preclinical imaging Biotechnology |
| Zdroj: | Bioconjugate chemistry. 31(5) |
| ISSN: | 1520-4812 |
| Popis: | Icosahedral virus-like particles (VLPs) derived from bacteriophages Qβ and PP7 encapsulating small-ultrared fluorescent protein (smURFP) were produced using a versatile supramolecular capsid disassemble-reassemble approach. The generated fluorescent VLPs display identical structural properties to their nonfluorescent analogs. Encapsulated smURFP shows indistinguishable photochemical properties to its unencapsulated counterpart, exhibits outstanding stability toward pH, and produces bright in vitro images following phagocytosis by macrophages. In vivo imaging allows the biodistribution to be imaged at different time points. Ex vivo imaging of intravenously administered encapsulated smURFP reveals a localization in the liver and kidneys after 2 h blood circulation and substantial elimination after 16 h of imaging, highlighting the potential application of these constructs as noninvasive in vivo imaging agents. |
| Databáze: | OpenAIRE |
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