Effects of Workers Exposure to Nanoparticles Studied by NMR Metabolomics
| Autor: | Štěpánka Vlčková, Daniela Pelclova, Štěpánka Dvořáčková, Jan Sýkora, Vladimír Ždímal, Lenka Michálková, Štěpán Horník |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
exhaled breath condensate
Technology QH301-705.5 QC1-999 Nanoparticle 02 engineering and technology Purine Metabolism Pathway 03 medical and health sciences NMR metabolomics Blood plasma General Materials Science Exhaled breath condensate Biology (General) nanoparticles exposure Instrumentation QD1-999 Nmr based metabolomics 030304 developmental biology Fluid Flow and Transfer Processes 0303 health sciences Chromatography human plasma Chemistry Process Chemistry and Technology Physics General Engineering 021001 nanoscience & nanotechnology Engineering (General). Civil engineering (General) Computer Science Applications Glutamine Metabolic pathway Proton NMR TA1-2040 0210 nano-technology |
| Zdroj: | Applied Sciences, Vol 11, Iss 6601, p 6601 (2021) Applied Sciences Volume 11 Issue 14 |
| ISSN: | 2076-3417 |
| Popis: | In this study, the effects of occupational exposure to nanoparticles (NPs) were studied by NMR metabolomics. Exhaled breath condensate (EBC) and blood plasma samples were obtained from a research nanoparticles-processing unit at a national research university. The samples were taken from three groups of subjects: samples from workers exposed to nanoparticles collected before and after shift, and from controls not exposed to NPs. Altogether, 60 1H NMR spectra of exhaled breath condensate (EBC) samples and 60 1H NMR spectra of blood plasma samples were analysed, 20 in each group. The metabolites identified together with binning data were subjected to multivariate statistical analysis, which provided clear discrimination of the groups studied. Statistically significant metabolites responsible for group separation served as a foundation for analysis of impaired metabolic pathways. It was found that the acute effect of NPs exposure is mainly reflected in the pathways related to the production of antioxidants and other protective species, while the chronic effect is manifested mainly in the alteration of glutamine and glutamate metabolism, and the purine metabolism pathway. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|