Enhanced osteogenesis of multilayered pore-closed microsphere-immobilized hydroxyapatite scaffold via sequential delivery of osteogenic growth peptide and BMP-2
| Autor: | Xin-guo Li, Lei He, Yandong Mu, Bingjun Zhang, Zhi-wei Han, Wei Zhi, Wei Zheng, Jie Weng |
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| Rok vydání: | 2020 |
| Předmět: |
Scaffold
Materials science Regeneration (biology) Biomedical Engineering Nanotechnology Osteoblast 02 engineering and technology General Chemistry General Medicine Bone healing 010402 general chemistry 021001 nanoscience & nanotechnology Bone tissue 01 natural sciences Bone morphogenetic protein 2 0104 chemical sciences Cell biology medicine.anatomical_structure Drug delivery medicine General Materials Science 0210 nano-technology Bone regeneration |
| Zdroj: | Journal of materials chemistry. B. 5(41) |
| ISSN: | 2050-7518 |
| Popis: | Since many complex physiological processes are controlled by multiple biomolecules, comprehensive regulation of bone tissue regeneration may be more effectively achieved by administering more than one type of biofactor. Thus, we propose a novel bone tissue engineering scaffold incorporating a multiple peptide-based drug delivery vehicle for accelerated bone regeneration. Pore-closed poly(lactic-co-glycolic acid) (PLGA) microspheres with a surface structure of multilayer polyelectrolytes ((Ha-Cs)2-Hep-BMP-2-Hep-(Cs-Ha)2) were prepared as multi-barrier microcarriers for osteogenic growth peptide (OGP). In addition, BMP-2 loading was achieved via a pore-closing process and layer-by-layer (LbL) assembly technique, followed by immobilization on the surface of a highly interconnected porous hydroxyapatite (HA) scaffold. On the basis of such a construction, sequential delivery of OGP and BMP-2 occurred in a coordinated manner through an orchestrated sequence of spatial changes, targeting different bone healing stages. The in vitro studies showed that OGP release was minimal ( |
| Databáze: | OpenAIRE |
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