The E75 gene of Manduca sexta and comparison with its Drosophila Homolog
| Autor: | Christine Woldin, William A. Segraves |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Ecdysone
animal structures Molecular Sequence Data Gene Expression Moths Biochemistry Homology (biology) Conserved sequence Species Specificity Sequence Homology Nucleic Acid Drosophilidae Melanogaster Animals Amino Acid Sequence Molecular Biology Gene Conserved Sequence Genetics Base Sequence Sequence Homology Amino Acid biology fungi Chromosome Mapping DNA biology.organism_classification Biological Evolution Drosophila melanogaster Manduca sexta Insect Science Manduca |
| Zdroj: | Insect Biochemistry and Molecular Biology. 23:91-97 |
| ISSN: | 0965-1748 |
| DOI: | 10.1016/0965-1748(93)90086-8 |
| Popis: | The ecdysone-inducible E75 gene responsible for the 75B puff of Drosophila melanogaster encodes a family of proteins which are members of the steroid receptor superfamily. These proteins are believed to be involved in the regulation of ecdysone response. In order to investigate the evolutionary conservation of E75, we have identified the E75 gene of Manduca sexta. We show here the structure of a cDNA believed to encode the Manduca homolog of the E75 B protein, and demonstrate that the putative DNA binding, hormone binding and amino and carboxy terminal flanking domains are conserved. However, due to a relative reduction in intron size and number and the absence of homopolymeric amino acid repeats, the E75 B transcription unit and protein are considerably smaller in M. sexta than in D. melanogaster. These findings have implications for the identification of critical structural features of E75 and also suggest that E75 has a conserved function and a shared ligand in Lepidoptera. |
| Databáze: | OpenAIRE |
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