Production of polyhydroxybutyrate in oil palm (Elaeis guineensis Jacq.) mediated by microprojectile bombardment of PHB biosynthesis genes into embryogenic calli
| Autor: | Nor Hanin Ayub, Bohari Bahariah, Ahmad Hashim Tarmizi, Zamzuri Ishak, Omar Abdul Rasid, Mat Yunus Abdul Masani, Ghulam Kadir Ahmad Parveez |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Genetically modified crops
Plant Science Biology lcsh:Plant culture Elaeis guineensis biology.organism_classification Nile blue oil palm Polyhydroxybutyrate Tissue culture chemistry.chemical_compound Micropropagation chemistry Callus biodegradable plastics Botany lcsh:SB1-1110 monocot biolistics Selectable marker Original Research transgenic |
| Zdroj: | Frontiers in Plant Science, Vol 6 (2015) Frontiers in Plant Science |
| ISSN: | 1664-462X |
| DOI: | 10.3389/fpls.2015.00598 |
| Popis: | Biodegradable plastics, mainly polyhydroxybutyrate (PHB), which are traditionally produced by bacterial cells, have been produced in the cells of more than 15 plant species. Since the production of biodegradable plastics and the synthesis of oil in plants share the same substrate, acetyl-coenzyme A (acetyl-CoA), producing PHB in oil bearing crops, such as oil palm, will be advantageous. In this study, three bacterial genes, bktB, phaB, and phaC, which are required for the synthesis of PHB and selectable marker gene, bar, for herbicide Basta resistant, were transformed into embryogenic calli. A number of transformed embryogenic lines resistant to herbicide Basta were obtained and were later regenerated to produce few hundred plantlets. Molecular analyses, including polymerase chain reaction (PCR), Southern blot, and real-time PCR have demonstrated stable integration and expression of the transgenes in the oil palm genome. HPLC and Nile blue A staining analyses confirmed the synthesis of PHB in some of the plantlets. |
| Databáze: | OpenAIRE |
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