RE: DETECTION OF CIRCULATING PROSTATE SPECIFIC ANTIGEN EXPRESSING PROSTATIC CELLS IN THE BONE MARROW OF RADICAL PROSTATECTOMY PATIENTS BY SENSITIVE REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION
| Autor: | F. Mannello, M. Malatesta, G. Gazzanelli, C.-L. Gao, R. C. Dean, A. Pinto, R. Mooneyhan, R. R. Connelly, D. G. McLeod, S. Srivastava, J. W. Moul |
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| Rok vydání: | 2000 |
| Předmět: |
Pathology
medicine.medical_specialty Prostatectomy business.industry Urology medicine.medical_treatment Reverse transcriptase Peripheral blood law.invention Prostate-specific antigen medicine.anatomical_structure law medicine Bone marrow Prostate cancer staging Stage (cooking) business Polymerase chain reaction |
| Zdroj: | Journal of Urology. 163:253-253 |
| ISSN: | 1527-3792 0022-5347 |
| DOI: | 10.1016/s0022-5347(05)68028-7 |
| Popis: | Purpose: The reverse transcriptase polymerase chain reaction (RT-PCR) assay for prostate specific antigen (PSA) expressing cells in the blood circulation has been under intense investigation since 1992. Although it has been suggested that this technology could be used as molecular staging for occult prostatic hematogenous metastases, we have been unable to confirm RT-PCR PSA positivity of peripheral blood to predict stage or recurrence in radical prostatectomy cases. We performed bone marrow RT-PCR PSA assay on a large cohort of radical prostatectomy cases and evaluate the use of this assay in improving prostate cancer staging and detecting early recurrence.Materials and Methods: Unilateral anterior iliac crest bone marrow aspirates were performed on 116 patients immediately before radical prostatectomy between February 1995 and September 1997. Radical prostatectomy specimens were processed as whole mounts. A sensitive nested RT-PCR assay with specific primers derived from the PSA sequence was use... |
| Databáze: | OpenAIRE |
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