Development of a technique to detect the activated form of the progesterone receptor and correlation with clinical and histopathological characteristics of endometrioid adenocarcinoma of the uterine corpus
| Autor: | Robert L. Coleman, Christine L. Clarke, Keiichi Fujiwara, Erard M. Gilles, Jacques Bonneterre, Jacques Bosq, Matthew A. Powell, Bradley J. Monk, Alexander Zukiwski, J. Dinny Graham, Emilie Hutt, Thomas J. Herzog, Eric Leblanc |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Pathology
medicine.medical_specialty animal structures Tissue Fixation medicine.drug_class Estrogen receptor Formaldehyde Progesterone receptor Medicine Humans Stage (cooking) Receptor Neoplasm Staging Paraffin Embedding business.industry Endometrial cancer Estrogen Receptor alpha Obstetrics and Gynecology medicine.disease Prognosis Immunohistochemistry Endometrial Neoplasms Ki-67 Antigen Oncology Hormonal therapy Female Neoplasm Grading business Receptors Progesterone Progestin Carcinoma Endometrioid |
| Zdroj: | Gynecologic oncology. 138(3) |
| ISSN: | 1095-6859 |
| Popis: | Objective Hormonal therapy is generally reserved for patients with endometrial cancers that fail cytotoxic chemotherapy, but there is a lack of sufficiently sensitive diagnostics to identify potential responders. We sought to develop a diagnostic technique to detect activated progesterone receptors (APR) in endometrial cancers using routine immunohistochemistry (IHC) and to correlate the presence of APR with other histopathological features and clinical disease stage. Methods Seventy-two tumor block specimens from patients with endometrial cancer were processed with conventional IHC methods for estrogen receptor-α (ERα), progesterone receptor (PR) and Ki67, a marker of proliferation. Tumor specimens were analyzed for the PR nuclear distribution patterns in individual tumor cells: APR positive (APR pos ) tumors were prospectively defined as any tumor with >5% countable malignant cells with an aggregated nuclear pattern. Tumor APR status was analyzed against other biomarkers including ERα expression, Ki67 and tumor grade. Results Fifty-six of 72 samples were endometrioid. Twenty-six of 49 PR-positive endometrioid tumors (53%; 95% CI 39–67%) were APR pos . Percent of ER pos cells correlated with % PR pos malignant cells (p=0.001, rho=0.44). APR positivity did not correlate with % PR pos cells in a given tumor, nor did it correlate with % Ki67 positivity; APR positivity was independent of disease stage and tumor grade (p=NS). Conclusions In this study, approximately half of endometrioid tumors were APR pos . APR is independent of histopathological and other known risk factors. Refining conventional PR detection has the potential to prospectively identify patients with endometrial cancer who may benefit from anti-progestin therapy. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect