D-Fructose Assimilation and Fermentation by Yeasts Belonging to Saccharomycetes: Rediscovery of Universal Phenotypes and Elucidation of Fructophilic Behaviors in Ambrosiozyma platypodis and Cyberlindnera americana
| Autor: | Moriya Ohkuma, Rikiya Endoh, Maiko Horiyama |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
ascomycetous yeasts Saccharomycetes Microbiology Article 03 medical and health sciences chemistry.chemical_compound Virology Food science Saccharomycotina fructophily lcsh:QH301-705.5 030304 developmental biology 0303 health sciences biology Ascomycota 030306 microbiology Kluyver rule physiological characterization Durham tube food and beverages Fructose Assimilation (biology) biology.organism_classification Yeast chemistry lcsh:Biology (General) physiology Fermentation |
| Zdroj: | Microorganisms Volume 9 Issue 4 Microorganisms, Vol 9, Iss 758, p 758 (2021) |
| ISSN: | 2076-2607 |
| Popis: | The purpose of this study was to investigate the ability of ascomycetous yeasts to assimilate/ferment d-fructose. This ability of the vast majority of yeasts has long been neglected since the standardization of the methodology around 1950, wherein fructose was excluded from the standard set of physiological properties for characterizing yeast species, despite the ubiquitous presence of fructose in the natural environment. In this study, we examined 388 strains of yeast, mainly belonging to the Saccharomycetes (Saccharomycotina, Ascomycota), to determine whether they can assimilate/ferment d-fructose. Conventional methods, using liquid medium containing yeast nitrogen base +0.5% (w/v) of d-fructose solution for assimilation and yeast extract-peptone +2% (w/v) fructose solution with an inverted Durham tube for fermentation, were used. All strains examined (n = 388, 100%) assimilated d-fructose, whereas 302 (77.8%) of them fermented d-fructose. In addition, almost all strains capable of fermenting d-glucose could also ferment d-fructose. These results strongly suggest that the ability to assimilate/ferment d-fructose is a universal phenotype among yeasts in the Saccharomycetes. Furthermore, the fructophilic behavior of Ambrosiozyma platypodis JCM 1843 and Cyberlindnera americana JCM 3592 was characterized by sugar consumption profiles during fermentation. |
| Databáze: | OpenAIRE |
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