Wild-type p53-induced phosphatase 1 (WIP1) regulates the proliferation of swine Sertoli cells through P53
| Autor: | Mingrui Zhang, Zhiguo Liu, Jingjing Che, Kui Li, Bingyuan Wang, Yulian Mu |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male endocrine system Swine Phosphatase Sertoli cell proliferation Reproductive technology Biology Gene Expression Regulation Enzymologic 03 medical and health sciences 0302 clinical medicine Endocrinology microRNA Genetics medicine Animals Phosphorylation Molecular Biology Cells Cultured Cell Proliferation Sertoli Cells Cell growth Wild type Sertoli cell Cell biology Protein Phosphatase 2C MicroRNAs 030104 developmental biology medicine.anatomical_structure Reproductive Medicine 030220 oncology & carcinogenesis Animal Science and Zoology Tumor Suppressor Protein p53 Spermatogenesis Protein Processing Post-Translational Developmental Biology Biotechnology |
| Zdroj: | Reproduction, fertility, and development. 32(18) |
| ISSN: | 1031-3613 |
| Popis: | Wild-type p53-induced phosphatase 1 (WIP1) plays an oncogenic function by increasing cell proliferation in various cancer types. Deficiency in WIP1 expression leads to male infertility, possibly by impairing the blood–testis barrier and spermatogenesis. However, how WIP1 functions in the Sertoli cells to affect male reproduction remains unclear. Thus, in the present study we used a swine Sertoli cell line to investigate whether WIP1 regulated the proliferation of Sertoli cells to participate in male reproduction. The WIP1 inhibitor GSK2830371, WIP1-short interference (si) RNAs and an upstream microRNA (miR-16) were used to inhibit the expression of WIP1, after which the proliferation of swine Sertoli cells, P53 expression and the levels of P53 phosphorylation were determined. Inhibiting WIP1 expression suppressed swine Sertoli cell proliferation, increased P53 expression and increased levels of P53 phosphorylation. In addition, overexpression of miR-16 in swine Sertoli cells resulted in a decrease in WIP1 expression and increases in both P53 expression and P53 phosphorylation. Together, these findings suggest that WIP1 positively regulates the proliferation of swine Sertoli cells by inhibiting P53 phosphorylation, and the miR-16 is likely also involved by targeting WIP1. |
| Databáze: | OpenAIRE |
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