Metabolomics Deciphered Metabolic Reprogramming Required for Biofilm Formation
Autor: | Haitao Lu, Hao Guo, Yumei Que, Tianbing Guan, Xia Wu |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
030106 microbiology lcsh:Medicine Carbohydrate metabolism Microbiology Article Mass Spectrometry 03 medical and health sciences Metabolomics Amino Acids lcsh:Science Pathogen Multidisciplinary biology lcsh:R Biofilm Metabolism biochemical phenomena metabolism and nutrition biology.organism_classification Plankton Phenotype Metabolic pathway 030104 developmental biology Biochemistry Biofilms Metabolome Carbohydrate Metabolism lcsh:Q Glycolipids Systems biology Bacteria Metabolic Networks and Pathways |
Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-7 (2019) |
ISSN: | 2045-2322 |
Popis: | Biofilm formation plays a key role in many bacteria causing infections, which mostly accounts for high-frequency infectious recurrence and antibiotics resistance. In this study, we sought to compare modified metabolism of biofilm and planktonic populations with UTI89, a predominant agent of urinary tract infection, by combining mass spectrometry based untargeted and targeted metabolomics methods, as well as cytological visualization, which enable us to identify the driven metabolites and associated metabolic pathways underlying biofilm formation. Surprisingly, our finding revealed distinct differences in both phenotypic morphology and metabolism between two patterns. Furthermore, we identified and characterized 38 differential metabolites and associated three metabolic pathways involving glycerolipid metabolism, amino acid metabolism and carbohydrate metabolism that were altered mostly during biofilm formation. This discovery in metabolic phenotyping permitted biofilm formation shall provide us a novel insight into the dissociation of biofilm, which enable to develop novel biofilm based treatments against pathogen causing infections, with lower antibiotic resistance. |
Databáze: | OpenAIRE |
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