The K+ and NO3− Interaction Mediated by NITRATE TRANSPORTER1.1 Ensures Better Plant Growth under K+-Limiting Conditions
| Autor: | Xian Zhi Fang, Ya Xing Zhu, Chong Wei Jin, Xing Xing Liu, Jia Yuan Ye |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Genotype Physiology Potassium Anion Transport Proteins Mutant Arabidopsis chemistry.chemical_element Plant Science Genes Plant Plant Roots 01 natural sciences Gene Expression Regulation Plant Genetics Arabidopsis thaliana Potassium Deficiency Research Articles Nitrates biology Arabidopsis Proteins Chemistry Genetic Variation Biological Transport Transporter Promoter biology.organism_classification Nitrogen Mutation Symporter Biophysics Plant Shoots 010606 plant biology & botany |
| Zdroj: | Plant Physiol |
| ISSN: | 1532-2548 0032-0889 |
| Popis: | K(+) and NO(3)(−) are the major forms of potassium and nitrogen that are absorbed by the roots of most terrestrial plants. In this study, we observed that a close relationship between NO(3)(−) and K(+) in Arabidopsis (Arabidopsis thaliana) is mediated by NITRATE TRANSPORTER1.1 (NRT1.1). The nrt1.1 knockout mutants showed disturbed K(+) uptake and root-to-shoot allocation, and were characterized by growth arrest under K(+)-limiting conditions. The K(+) uptake and root-to-shoot allocation of these mutants were partially recovered by expressing NRT1.1 in the root epidermis-cortex and central vasculature using SULFATE TRANSPORTER1;2 and PHOSPHATE1 promoters, respectively. Two-way analysis of variance based on the K(+) contents in nrt1.1-1/K(+) transporter1, nrt1.1-1/high-affinity K(+) transporter5-3, nrt1.1-1/K(+) uptake permease7, and nrt1.1-1/stelar K(+) outward rectifier-2 double mutants and the corresponding single mutants and wild-type plants revealed physiological interactions between NRT1.1 and K(+) channels/transporters located in the root epidermis–cortex and central vasculature. Further study revealed that these K(+) uptake-related interactions are dependent on an H(+)-consuming mechanism associated with the H(+)/NO(3)(−) symport mediated by NRT1.1. Collectively, these data indicate that patterns of NRT1.1 expression in the root epidermis–cortex and central vasculature are coordinated with K(+) channels/transporters to improve K(+) uptake and root-to-shoot allocation, respectively, which in turn ensures better growth under K(+)-limiting conditions. |
| Databáze: | OpenAIRE |
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