Sororin actively maintains sister chromatid cohesion
| Autor: | Maria Helena Idarraga-Amado, Emanuel Kreidl, David A. Cisneros, Rene Ladurner, Gordana Wutz, Heinz Ekker, Miroslav P Ivanov, Jan-Michael Peters, Georg A. Busslinger |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Embryonic Development Cell Cycle Proteins cohesin acetylation Biology Chromatids General Biochemistry Genetics and Molecular Biology Article ESCO2 Chromosome segregation 03 medical and health sciences Mice 0302 clinical medicine Acetyltransferases Chromosome Segregation Sister chromatids Animals Molecular Biology Adaptor Proteins Signal Transducing Genetics mitosis General Immunology and Microbiology Cohesin General Neuroscience DNA Replication Repair & Recombination Articles Chromatin Cell biology sister chromatid cohesion Establishment of sister chromatid cohesion 030104 developmental biology Chromatid cell cycle Separase biological phenomena cell phenomena and immunity 030217 neurology & neurosurgery |
| Zdroj: | The EMBO Journal |
| ISSN: | 1460-2075 |
| Popis: | Cohesion between sister chromatids is established during DNA replication but needs to be maintained to enable proper chromosome–spindle attachments in mitosis or meiosis. Cohesion is mediated by cohesin, but also depends on cohesin acetylation and sororin. Sororin contributes to cohesion by stabilizing cohesin on DNA. Sororin achieves this by inhibiting WAPL, which otherwise releases cohesin from DNA and destroys cohesion. Here we describe mouse models which enable the controlled depletion of sororin by gene deletion or auxin‐induced degradation. We show that sororin is essential for embryonic development, cohesion maintenance, and proper chromosome segregation. We further show that the acetyltransferases ESCO1 and ESCO2 are essential for stabilizing cohesin on chromatin, that their only function in this process is to acetylate cohesin's SMC3 subunit, and that DNA replication is also required for stable cohesin–chromatin interactions. Unexpectedly, we find that sororin interacts dynamically with the cohesin complexes it stabilizes. This implies that sororin recruitment to cohesin does not depend on the DNA replication machinery or process itself, but on a property that cohesin acquires during cohesion establishment. |
| Databáze: | OpenAIRE |
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