Antibody-modified iron oxide nanoparticles for efficient magnetic isolation and flow cytometric determination of L. pneumophila
| Autor: | Miet Peeters, Nick Geukens, Maarten Bloemen, Ann Gils, Luc De Meester, Thierry Verbiest, Carla Denis |
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| Rok vydání: | 2015 |
| Předmět: |
Chromatography
biology medicine.diagnostic_test Chemistry biology.organism_classification Ligand (biochemistry) Legionella pneumophila Combinatorial chemistry Analytical Chemistry law.invention Green fluorescent protein Flow cytometry chemistry.chemical_compound law Polyclonal antibodies Recombinant DNA biology.protein medicine Ethylene glycol Iron oxide nanoparticles |
| Zdroj: | Microchimica Acta. 182:1439-1446 |
| ISSN: | 1436-5073 0026-3672 |
| DOI: | 10.1007/s00604-015-1466-z |
| Popis: | © 2015, Springer-Verlag Wien. We report on the design of superparamagnetic nanoparticles capable of selectively isolating targeted bacteria (Legionella pneumophila, serogroup 1) from aqueous solutions. The surface of magnetite nanoparticles (NP) was functionalized with a heterobifunctional poly(ethylene glycol) ligand containing reactive groups for covalent coupling of polyclonal antibodies against L. pneumophila. These bioconjugates were used to label and magnetically isolate L. pneumophila. Flow cytometry revealed high separation and efficiency in this regard. The strain specificity and efficiency of the magnetic NP was tested with recombinant strains of E. coli (expressing the red fluorescent protein) and L. pneumophila (expressing the green fluorescent protein). The detection limit of the method (by flow cytometry) is 104 cells∙mL-1. The results indicate that the new multifunctional NPs are capable of selectively attracting pathogens from a complex mixture and with high efficiency. This, conceivably, paves the way to pre-concentration protocols for numerous other pathogens. [Figure not available: see fulltext.] ispartof: Microchimica Acta vol:182 issue:7 pages:1439-1446 status: published |
| Databáze: | OpenAIRE |
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