Antibiotic-free expression system for the production of human interferon-beta protein
Autor: | Uttam Chand Banerjee, Madaka Surya Teja, Mukesh Kumar, Abhay H. Pande, Dharam Pal, Rajan K. Tripathy |
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Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
0106 biological sciences
0301 basic medicine medicine.drug_class 030106 microbiology Antibiotics Environmental Science (miscellaneous) medicine.disease_cause 01 natural sciences law.invention Microbiology 03 medical and health sciences Interferon law 010608 biotechnology medicine Bioreactor Human Interferon Beta Escherichia coli Chemistry Agricultural and Biological Sciences (miscellaneous) Yield (chemistry) Recombinant DNA bacteria Original Article Stem cell Biotechnology medicine.drug |
Popis: | Recombinant human interferon-β (rhIFN-β), a therapeutic protein, is produced using both prokaryotic and eukaryotic expression systems. However, instability of recombinant plasmid during cultivation of Escherichia coli results in low yield of the recombinant proteins. In addition, use of antibiotics during the cultivation imposes a major concern. In this study, we have compared the expression yield of rhIFN-β in E. coli BL21 (DE3) and E coli SE1 cells. Gene-encoding rhIFN-β was expressed in E. coli BL21 (DE3) and SE1 cells and the cultivation of recombinant E. coli cells was done in a laboratory scale bioreactor. Our results suggest that, compared to BL21(DE3) cells, the SE1 cells expressing rhIFN-β protein can be cultivated in the medium without antibiotic and provide increased stability of recombinant plasmid and higher expression yield of rhIFN-β protein. This system can be used for the production of rhIFN-β proteins for biomedical applications. |
Databáze: | OpenAIRE |
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