The endogenous transposable element Tgm9 is suitable for generating knockout mutants for functional analyses of soybean genes and genetic improvement in soybean

Autor: Jayadri Sekhar Ghosh, Callie Johnson, Madan K. Bhattacharyya, Eric Baumert, Tyler Cina, Devinder Sandhu, Jordan Baumbach, Reid G. Palmer, David Grant
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0106 biological sciences
0301 basic medicine
Mutant
lcsh:Medicine
Artificial Gene Amplification and Extension
01 natural sciences
Polymerase Chain Reaction
Exon
Gene Knockout Techniques
Mathematical and Statistical Techniques
Mobile Genetic Elements
lcsh:Science
Plant Proteins
Genetics
Multidisciplinary
Insertion Mutation
Chromosome Biology
food and beverages
Agriculture
Genomics
Plants
Genetically Modified
Research Article
Transposable element
Locus (genetics)
Crops
Biology
Genome Complexity
Research and Analysis Methods
Genes
Plant
Chromosomes
Chromosomes
Plant
03 medical and health sciences
Genetic Elements
Insertion
Allele
Molecular Biology Techniques
Gene
Molecular Biology
Alleles
fungi
lcsh:R
Intron
Transposable Elements
Biology and Life Sciences
Computational Biology
Cell Biology
Introns
Alcohol Oxidoreductases
030104 developmental biology
Genetic Loci
Mutation
DNA Transposable Elements
lcsh:Q
Soybeans
Soybean
Functional Analysis
010606 plant biology & botany
Crop Science
Zdroj: PLoS ONE, Vol 12, Iss 8, p e0180732 (2017)
PLoS ONE
ISSN: 1932-6203
Popis: In soybean, variegated flowers can be caused by somatic excision of the CACTA-type transposable element Tgm9 from Intron 2 of the DFR2 gene encoding dihydroflavonol-4-reductase of the anthocyanin pigment biosynthetic pathway. DFR2 was mapped to the W4 locus, where the allele containing Tgm9 was termed w4-m. In this study we have demonstrated that previously identified morphological mutants (three chlorophyll deficient mutants, one male sterile-female fertile mutant, and three partial female sterile mutants) were caused by insertion of Tgm9 following its excision from DFR2. Analyses of Tgm9 insertion sites among 105 independent mutants demonstrated that Tgm9 hops to all 20 soybean chromosomes from its original location on Chromosome 17. Some genomic regions are prone to increased Tgm9-insertions. Tgm9 transposed over 25% of the time into exon or intron sequences. Tgm9 is therefore suitable for generating an indexed insertional mutant collection for functional analyses of most soybean genes. Furthermore, desirable Tgm9-induced stable knockout mutants can be utilized in generating improved traits for commercial soybean cultivars.
Databáze: OpenAIRE
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