Determination of antioxidants in bovine oviduct epithelial cell culture isolated at different periods of the estrous cycle
| Autor: | Hakan Kocamiş, Aytül Kürüm, Siyami Karahan, Miyase Çinar, Emel Ergün |
|---|---|
| Přispěvatelé: | Kırıkkale Üniversitesi |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Estrous cycle
animal structures General Veterinary biology urogenital system catalase Luteal phase Ampulla bovine oviduct epithelial cell catalase glutathione peroxidase isthmus superoxide dismutase superoxide dismutase Ampulla Superoxide dismutase Andrology Human fertilization Capacitation bovine oviduct epithelial cell isthmus Gamete transport biology.protein Oviduct glutathione peroxidase |
| Zdroj: | Volume: 43, Issue: 4 448-455 Turkish Journal of Veterinary and Animal Sciences |
| ISSN: | 1300-0128 1303-6181 |
| Popis: | Karahan, Siyami/0000-0002-2744-1717 WOS: 000479121700003 Oxidative stress interferes with oviduct functions including oocyte maturation, capacitation, fertilization, and embryo and gamete transport. This study aimed to determine activity of the antioxidants glutathione peroxidase (GPX-I), superoxide dismutase (SOD), and catalase (CAT) in bovine oviduct epithelial cells (BOEC) isolated from the isthmus and ampulla of the oviduct at estral (n = 7) and luteal phases (n = 7) of the estrous cycle. The antioxidant activity was measured at the primary, first, and second passages of the cell culture, and was characterized by cytokeratin expression. The GPX activity increased over the passages in samples of the ampulla and the isthmus of each sexual phase without statistical significance. The SOD activity remained steady through the cell passages in both sexual phases. CAT activity at the primary culture was higher in the ampulla compared to the isthmus in both sexual phases with a significant difference for the estral phase (P < 0.05), and it decreased over the passages with no significant differences. In conclusion, the antioxidant enzyme activity profile of BOEC did not differ by region or sexual cycle except for that of CAT, which was higher in the ampulla. Further studies should focus on SOD, GPX, and CAT activity for the mechanism of BOEC adaptation to an in vitro environment. Kirikkale University Scientific Research Coordination UnitKirikkale University [2013/81] This study was supported by Kirikkale University Scientific Research Coordination Unit (Project no. 2013/81). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|