An immunoassay for TEQ screening of dioxin/furan samples: Current status of assay and applications development
| Autor: | Robert O. Harrison, Robert E. Carlson |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Polychlorinated Dibenzodioxins
Environmental Engineering Chromatography Anticorps monoclonal medicine.diagnostic_test Chemistry Health Toxicology and Mutagenesis Public Health Environmental and Occupational Health General Medicine General Chemistry Dioxins Sensitivity and Specificity Pollution Immunoenzyme Techniques Congener Mouse monoclonal antibody Immunoassay Toxicity Tests medicine Soil Pollutants Environmental Chemistry Furans |
| Zdroj: | Chemosphere. 34:915-928 |
| ISSN: | 0045-6535 |
| DOI: | 10.1016/s0045-6535(97)00395-0 |
| Popis: | A simple and easy to use system has been developed for rapid screening of 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) in a variety of situations. The system uses a competitive inhibition Enzyme ImmunoAssay (EIA) based on a previously described mouse monoclonal antibody which is specific for 2,3,7,8-TCDD and related congeners. Two formats have been developed, a rapid tube test and a microplate test. Respective sensitivities are 100 pg/tube and 25 pg/well of 2,3,7,8-TCDD. Congener specificity of the tube test roughly correlates with 1-TEF. The concept of TEQ screening is validated by comparing TEQ values for a set of soil samples to the EIA responses predicted for those samples. Actual analysis of crude extracts of some of the same soils indicated some clean-up is required to remove interferences. Rapid extraction and sample clean-up methods for soil and other matrices are being developed. Potential implementation of the EIA in a fixed lab is discussed. The EIA system offers significant improvements in speed, sample throughput, and cost compared to GC-MS. This system should be useful for screening environmental samples in many situations. |
| Databáze: | OpenAIRE |
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