Recovery of liver function in two-third partial hepatectomized rats evaluated by L-[1-13C]phenylalanine breath test
| Autor: | Asuka Ito, Tadashi Kohno, Koichi Ishikawa, Satoshi Asai, Yukimoto Ishii, Shigetomi Iwai |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Male
medicine.medical_specialty Paha Phenylalanine medicine.medical_treatment Femoral vein Sodium pentobarbital Liver weight Liver Function Tests Internal medicine medicine Animals Hepatectomy Rats Wistar Breath test Carbon Isotopes medicine.diagnostic_test business.industry Body Weight Phenylalanine Hydroxylase Proteins DNA Organ Size Recovery of Function Liver Regeneration Rats Endocrinology Breath Tests Liver Surgery Liver function business |
| Zdroj: | Surgery. 132:849-856 |
| ISSN: | 0039-6060 |
| DOI: | 10.1067/msy.2002.127947 |
| Popis: | Background. We have previously reported that by means of a breath test with intravenously administered L-[1- 13 C] phenylalanine ( 13 Cphe), hepatopathy could be quantitatively evaluated by measuring expiratory 13 CO 2 levels in a short period. It is known that phenylalanine hydroxylase activity (PAHA) plays an important role in phenylalanine metabolism. We examined the relationship between changes in PAHA and the results of the 13 Cphe breath test during hepatic regeneration in a rat model of 70% hepatectomy, to assess their usefulness for evaluating hepatic regeneration. Methods. Male Wistar rats (Shizvoka Laboratory Animal Center, Hamamatsu, Japan) weighing 230 to 290 g were subjected to 70% hepatectomy under anesthesia with sodium pentobarbital. One, 2, 3, 5, 7, and 14 days postoperatively, 30 mg/kg 13 Cphe was intravenously injected into the femoral vein, and the increase in exhaled 13 CO 2 (Δ 13 CO 2 ) was measured for 15 minutes. Simple laparotomy was performed in control rats. After the breath test, the regenerated liver was removed and weighed. The amount of DNA, amount of hepatic tissue total protein (TP), and PAHA were determined. Results. The r between liver weight/body weight and PAHA, between DNA and PAHA, and between TP and PAHA were 0.832, 0.720, and 0.758, respectively. Breath test graphs revealed that liver weight/body weight, DNA, and TP showed the best correlations with the peak value of Δ 13 CO 2 (liver weight/body weight percentage, r = 0.801; DNA, r = 0.660; TP, r = 0.706), and r between PAHA and peak value was 0.638. Conclusions. These results suggest that measurement of PAHA in regenerated liver is an effective method for following up liver function after hepatic resection. Moreover, the 13 Cphe breath test may also be useful to evaluate liver function after partial hepatectomy. Surgery 2002;132:849-56. |
| Databáze: | OpenAIRE |
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