The effectiveness of platelet-rich plasma gel on full-thickness cartilage defect repair in a rabbit model
| Autor: | Hassib Keskes, Raja Amri, Wassim Zribi, Samira Jebahi, Mohammed Ali Rebai, N. Gouiaa, Tahia Boudawara, Moez Trigui, Fathia Slimi, Cyrine Abid |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
030222 orthopedics
Defect repair PRP lcsh:Diseases of the musculoskeletal system Histological study Immunohistochemical study Chemistry Regeneration (biology) Cartilage Rabbit knee 030229 sport sciences Osteochondral 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure Platelet-rich plasma Rabbit model medicine Orthopedics and Sports Medicine Surgery Full thickness Defect lcsh:RC925-935 Biomedical engineering |
| Zdroj: | Bone & Joint Research Bone & Joint Research, Vol 10, Iss 3, Pp 192-202 (2021) |
| ISSN: | 2046-3758 |
| Popis: | Aims The present study investigates the effectiveness of platelet-rich plasma (PRP) gel without adjunct to induce cartilage regeneration in large osteochondral defects in a rabbit model. Methods A bilateral osteochondral defect was created in the femoral trochlear groove of 14 New Zealand white rabbits. The right knees were filled with PRP gel and the contralateral knees remained untreated and served as control sides. Some animals were killed at week 3 and others at week 12 postoperatively. The joints were harvested and assessed by Magnetic Resonance Observation of Cartilage Repair Tissue (MOCART) MRI scoring system, and examined using the International Cartilage Repair Society (ICRS) macroscopic and ICRS histological scoring systems. Additionally, the collagen type II content was evaluated by the immunohistochemical staining. Results After 12 weeks post-surgery, the defects of the PRP group were repaired by hyaline cartilage-like tissue. However, incomplete cartilage regeneration was observed in the PRP group for three weeks. The control groups showed fibrocartilaginous or fibrous tissue, respectively, at each timepoint. Conclusion Our study proved that the use of PRP gel without any adjuncts could successfully produce a good healing response and resurface the osteochondral defect with a better quality of cartilage in a rabbit model. Cite this article: Bone Joint Res 2021;10(3):192–202. |
| Databáze: | OpenAIRE |
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