Serotonergic neurons in the nucleus raphé obscurus are not involved in the ventilatory and thermoregulatory responses to hypoxia in adult rats
| Autor: | Olagide W. Castro, Humberto Giusti, Luiz G.S. Branco, Mogens L. Glass, Glauber S. F. da Silva, Luciane H. Gargaglioni, Norberto Garcia-Cairasco |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
Pulmonary and Respiratory Medicine Serotonin medicine.medical_specialty Time Factors Microinjections Physiology Cell Count Biology Serotonergic Antibodies Nucleus raphe obscurus Body Temperature Lesion chemistry.chemical_compound Internal medicine Excitatory Amino Acid Agonists medicine Animals Rats Wistar Hypoxia Ibotenic Acid Plethysmography Whole Body Raphe General Neuroscience RNA-Binding Proteins Thermoregulation Rats Disease Models Animal Endocrinology chemistry MODELOS ANIMAIS Anesthesia Breathing Raphe Nuclei Blood Gas Analysis medicine.symptom Pulmonary Ventilation Ibotenic acid Body Temperature Regulation Serotonergic Neurons |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1569-9048 |
| DOI: | 10.1016/j.resp.2013.04.008 |
| Popis: | The medullary raphé is an important component of the central respiratory network, playing a key role in CO2 central chemoreception. However, its participation in hypoxic ventilatory responses is less understood. In the present study, we assessed the role of nucleus raphé obscurus (ROb), and specifically 5-HT neurons confined in the ROb, on ventilatory and thermoregulatory responses to hypoxia. Chemical lesions of the ROb were performed using either ibotenic acid (non-specific lesion; control animals received PBS) or anti-SERT-SAP (5-HT specific lesion; control animals received IgG-SAP). Ventilation (V˙E; whole body plethysmograph) and body temperature (Tb; data loggers) were measured during normoxia (21% O2, N2 balance) and hypoxia exposure (7% O2, N2 balance, 1h) in conscious adult rats. Ibotenic acid or anti-SERT-SAP-induced lesions did not affect baseline values of V˙E and Tb. Similarly, both lesion procedures did not alter the ventilatory or thermoregulatory responses to hypoxia. Although evidence in the literature suggests a role of the rostral medullary raphé in hypoxic ventilatory responses, under the present experimental conditions our data indicate that caudal medullary raphé (ROb) and its 5-HT neurons neither participate in the tonic maintenance of breathing nor in the ventilatory and thermal responses to hypoxia. |
| Databáze: | OpenAIRE |
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