Red light-triggered nucleic acid-templated reaction based on cyclic oligonucleotide substrates

Autor:	Frank R. Beierlein, Tobias Bachmann, Mike Heilemann, Andriy Mokhir, Nina S. Deussner-Helfmann, Oleksii Zozulia
Rok vydání:	2019
Předmět:	Light Molecular Structure 010405 organic chemistry Chemistry Oligonucleotide Oligonucleotides Metals and Alloys Fluorescence correlation spectroscopy General Chemistry 010402 general chemistry 01 natural sciences Fluorescence Combinatorial chemistry Catalysis 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Spectrometry Fluorescence Template Nucleic Acids Materials Chemistry Ceramics and Composites Nucleic acid Red light Fluorescent Dyes
Zdroj:	Chemical Communications. 55:10713-10716
ISSN:	1364-548X 1359-7345
Popis:	A red light-triggered reaction based on cyclic oligonucleotide substrates that is accelerated over 30-fold by specific nucleic acid templates and generates a bright fluorescent probe was developed. We confirmed that this reaction is compatible with fluorescence correlation spectroscopy (FCS) thereby allowing detection of nucleic acids down to 1 nM.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::96a0c85065c032e9f895f7702c6a13e7 https://doi.org/10.1039/c9cc03587g Zobrazit plný text záznamu