Autoradiographic localization of leptin binding in the choroid plexus of ob/ob and db/db mice
| Autor: | Robert V. Considine, Richard B. Lynn, Thomas M. Hyde, Gao Yuan Cao, Jose F. Caro |
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| Rok vydání: | 1996 |
| Předmět: |
Leptin
medicine.medical_specialty Biophysics Adipose tissue Mice Obese Receptors Cell Surface Biology Biochemistry Binding Competitive Iodine Radioisotopes Diabetes mellitus genetics Lateral ventricles Mice Species Specificity Internal medicine medicine Diabetes Mellitus Animals Obesity Receptors Cytokine Receptor Molecular Biology Third ventricle Leptin receptor Binding Sites digestive oral and skin physiology Brain Proteins Cell Biology Mice Mutant Strains Mice Inbred C57BL Kinetics medicine.anatomical_structure Endocrinology Autoradiography Receptors Leptin Choroid plexus Female Carrier Proteins hormones hormone substitutes and hormone antagonists |
| Zdroj: | Biochemical and biophysical research communications. 219(3) |
| ISSN: | 0006-291X |
| Popis: | The obese gene product, leptin, is synthesized in adipose tissue and is a circulating factor regulating body weight. To identify the location of leptin receptors in the brain we have performed an autoradiographic study of the binding of [(125)I]leptin to frozen sections of mouse brain. Dense specific binding of [(125)I]leptin was found only in the choroid plexus which is located in the dorsal part of the third ventricle and lateral ventricles. Specific binding of [(125)I]leptin was found the ob/ob and db/db mice. These findings further our understanding of the sites and mechanism of action of leptin on brain centers regulating body weight. |
| Databáze: | OpenAIRE |
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