DogMATIC—A Remote Biospecimen Collection Kit for Biobanking
| Autor: | Samantha J. Richardson, Kristi M Milley, Judith S Nimmo, Barbara Bacci, Janine A. Danks, Stewart D. Ryan |
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| Přispěvatelé: | Milley, Kristi M., Nimmo, Judith S., Bacci, Barbara, Ryan, Stewart D., Richardson, Samantha J., Danks, Janine A. |
| Rok vydání: | 2015 |
| Předmět: |
Male
Rural Population Veterinary Medicine Pathology medicine.medical_specialty Victoria Veterinary clinics Veterinary pathology Medicine (miscellaneous) Pilot Projects Cancer Biobank Tissue Banks Biospecimen Collection General Biochemistry Genetics and Molecular Biology Specimen Handling Dogs Tissue Bank Neoplasms Biological Specimen Bank Dog Animals Medicine Pilot Project Biological Specimen Banks Quality of Health Care Biochemistry Genetics and Molecular Biology (all) Animal business.industry Cell Biology General Medicine medicine.disease Biobank Sample quality Disease Models Animal Tissue bank Neoplasm RNA Female Medical emergency business |
| Zdroj: | Biopreservation and Biobanking. 13:247-254 |
| ISSN: | 1947-5543 1947-5535 |
| DOI: | 10.1089/bio.2014.0085 |
| Popis: | Canine tumors are valuable comparative oncology models. This research was designed to create a sustainable biobank of canine mammary tumors for breast cancer research. The aim was to provide a well-characterized sample cohort for specimen sharing, data mining, and long-term research aims. Canine mammary tumors are most frequently managed at a local veterinary clinic or hospital. We adopted a biobank framework based on a large number of participating veterinary hospitals and clinics acting as collection centers that were serviced by a centralized storage facility. Recruitment was targeted at rural veterinary clinics. A tailored, stable collection kit (DogMATIC) was designed that was used by veterinarians in remote or rural locations to collect both fresh and fixed tissue for submission to the biobank. To validate this methodology the kit design, collection rate, and sample quality were analyzed. The Australian Veterinary Cancer Biobank was established as a network of 47 veterinary clinics and three veterinary pathology laboratories spanning over 200,000 km2. In the first 12 months, 30 canine mammary tumor cases were submitted via the DogMATIC kit. Pure intact RNA was isolated in over 80% of samples with an average yield of 14.49 μg. A large network biobank, utilizing off-site collection with the DogMATIC kit, was successfully coordinated. The creation of the Australian Veterinary Cancer Biobank has established a long-term, sustainable, comparative oncology research resource in Australia. There are broader implications for biobanking with this very different form of collection and banking. |
| Databáze: | OpenAIRE |
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