Effect of expression of Jagged1 protein in stromal sublayer on the maintenance of hemopoietic precursors in in vitro coculturing system
| Autor: | A. V. Belyavskii, F. N. Rozov |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Myeloid
Stromal cell Immunoblotting Clone (cell biology) Bone Marrow Cells Biology Transfection General Biochemistry Genetics and Molecular Biology Colony-Forming Units Assay Mice medicine Animals Serrate-Jagged Proteins Fibroblast Cells Cultured Cell Proliferation Cell growth Calcium-Binding Proteins Membrane Proteins General Medicine Fibroblasts Hematopoietic Stem Cells Coculture Techniques In vitro Hematopoiesis Cell biology Mice Inbred C57BL Haematopoiesis medicine.anatomical_structure Immunology NIH 3T3 Cells Intercellular Signaling Peptides and Proteins Bone marrow Stromal Cells Jagged-1 Protein |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 143:514-518 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/s10517-007-0169-9 |
| Popis: | In order to verify the relationship between the expression of Notch ligand in NIH 3T3 fibroblast clones and maintenance of hemopoietic activity in vitro, monolayers from three clones were co-cultured with mouse bone marrow mononuclears for 1-4 weeks and the total production of hemopoietic cells and myeloid colony-forming activity in methylcellulose were evaluated. One of the clones exhibited significant hemopoiesis maintaining activity and after 2 weeks of culturing promoted a more than 100-fold increase in the number of colony-forming precursors in comparison with control cells. Hemopoiesis maintenance activity of the second clone was much lower, and that of the third clone virtually did not differ from that of control cells. The results indicate that expression of Notch ligand can radically increase cell strain capacity to maintenance of in vitro hemopoiesis in the co-culturing system. |
| Databáze: | OpenAIRE |
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