Biosensor immunoassay for flumequine in broiler serum and muscle

Autor: Hüsniye Gerçek, Geert Cazemier, Willem Haasnoot, Michel W. F. Nielen
Jazyk: angličtina
Rok vydání: 2007
Předmět:
Maximum Residue Limit
RIKILT - Business Unit Veiligheid & Gezondheid
Enzyme-Linked Immunosorbent Assay
sarafloxacin
Biosensing Techniques
Biochemistry
Mass Spectrometry
Analytical Chemistry
residues
Blood serum
Sarafloxacin
Anti-Infective Agents
ciprofloxacin
medicine
Enrofloxacin
Environmental Chemistry
Animals
antibodies
fluoroquinolones
Spectroscopy
BU Microbiological & Chemical Food Analysis
Immunoassay
Chromatography
biology
medicine.diagnostic_test
Chemistry
Muscles
Broiler
indirect competitive elisa
tissue
Surface Plasmon Resonance
Drug Residues
products
Models
Chemical
Polyclonal antibodies
Flumequine
biology.protein
RIKILT - Business Unit Safety & Health
Rabbits
BU Microbiologische & Chemische Voedselanalyse
enrofloxacin
linked-immunosorbent-assay
Chickens
Food Analysis
medicine.drug
Chromatography
Liquid
Zdroj: Analytica Chimica Acta 586 (2007) 1-2
Analytica Chimica Acta, 586(1-2), 312-318
ISSN: 0003-2670
Popis: Flumequine (Flu) is one of the fluoroquinolones most frequently applied for the treatment of broilers in The Netherlands. For the detection of residues of Flu in blood serum of broilers, a biosensor immunoassay (BIA) was developed which was fast (7.5 min per sample) and specific (no cross-reactivity with other (fluoro)quinolones). This inhibition assay was based on a rabbit polyclonal anti-Flu serum and a CM5 biosensor chip coated with Flu which could be detected in the range of 15-800 ng mL(-1). For the detection of Flu in muscle, an easy extraction procedure in buffer was selected and the measuring range was from 24 to 4000 ng g(-1). Average recoveries of 66 till 75% were found with muscle samples spiked at 0.5, 1 and 2 times the maximum residue limit (MRL in muscle = 400 ng g(-1)) and the decision limit (CC alpha) and the detection capability (CC beta) were determined as 500 and 600 ng respectively. Incurred muscle samples were analysed by the BIA and by LC-MS/MS and a good correlation was found (R-2 = 0.998). Serum and muscle samples from with Flu treated broilers were analysed and the concentrations found in serum were always higher than those found in muscle (average serum/muscle ratio was 3.5) and this proved the applicability of the BIA in serum as predictor of the Flu concentration in muscle. (c) 2006 Elsevier B.V. All rights reserved.
Databáze: OpenAIRE
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