Perfusion culture of multi-layered HepG2 hepatocellular carcinoma cells in a pressure-driven microphysiological system

Autor:	Shinji Sugiura, Taku Satoh, Kazumi Shin, Reiko Onuki-Nagasaki, Toshiyuki Kanamori
Rok vydání:	2022
Předmět:	Perfusion Carcinoma Hepatocellular Albumins Liver Neoplasms Cell Culture Techniques Hepatocytes Humans Urea Bioengineering Hep G2 Cells Applied Microbiology and Biotechnology Biotechnology
Zdroj:	Journal of Bioscience and Bioengineering. 134:348-355
ISSN:	1389-1723
Popis:	Here we report the perfusion culture of a multi-layered tissue composed of HepG2 cells (a human hepatoma line) in a pressure-driven microphysiological system (PD-MPS), which we developed previously as a multi-throughput perfusion culture platform. The perfusion culture of multi-layered tissue model was constructed by inserting a modified commercially available permeable membrane insert into the PD-MPS. HepG2 cells were layered on the membrane, and culture medium was perfused both through and below the membrane. The seeded density (number of cells/cm
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::969605442b52d7dcff9216c58d0eedfd https://doi.org/10.1016/j.jbiosc.2022.07.001 Zobrazit plný text záznamu Full Text from ScienceDirect