Genetic and metabolic effects of ripening mutations and vine detachment on tomato fruit quality
| Autor: | Ryan P. McQuinn, James J. Giovannoni, José G. Vallarino, Sonia Osorio, Alisdair R. Fernie, Raphael Tozelli Carneiro, Iben Sørensen, Anna Lytovchenko, Jocelyn K. C. Rose |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Sucrose Plant Science Biology 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Solanum lycopersicum Gene Expression Regulation Plant Cultivar Carotenoid Research Articles Plant Proteins mutants 2. Zero hunger chemistry.chemical_classification fruit quality Primary metabolite food and beverages Fructose Ripening Ethylenes biology.organism_classification tomato ripening Horticulture 030104 developmental biology chemistry Fruit Mutation Postharvest Solanum Agronomy and Crop Science 010606 plant biology & botany Biotechnology Research Article |
| Zdroj: | Plant Biotechnology Journal |
| ISSN: | 1467-7652 |
| Popis: | Summary Tomato (Solanum lycopersicum) fruit ripening is regulated co‐operatively by the action of ethylene and a hierarchy of transcription factors, including RIPENING INHIBITOR (RIN) and NON‐RIPENING (NOR). Mutations in these two genes have been adopted commercially to delay ripening, and accompanying textural deterioration, as a means to prolong shelf life. However, these mutations also affect desirable traits associated with colour and nutritional value, although the extent of this trade‐off has not been assessed in detail. Here, we evaluated changes in tomato fruit pericarp primary metabolite and carotenoid pigment profiles, as well as the dynamics of specific associated transcripts, in the rin and nor mutants during late development and postharvest storage, as well of those of the partially ripening delayed fruit ripening (dfd) tomato genotype. These profiles were compared with those of the wild‐type tomato cultivars Ailsa Craig (AC) and M82. We also evaluated the metabolic composition of M82 fruit ripened on or off the vine over a similar period. In general, the dfd mutation resulted in prolonged firmness and maintenance of quality traits without compromising key metabolites (sucrose, glucose/fructose and glucose) and sectors of intermediary metabolism, including tricarboxylic acid cycle intermediates. Our analysis also provided insights into the regulation of carotenoid formation and highlighted the importance of the polyamine, putrescine, in extending fruit shelf life. Finally, the metabolic composition analysis of M82 fruit ripened on or off the vine provided insights into the import into fruit of compounds, such as sucrose, during ripening. |
| Databáze: | OpenAIRE |
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