Cytoskeletal Regulation by the Nedd8 Ubiquitin-Like Protein Modification Pathway

Autor: John H. Willis, Matthias Peter, Bruce Bowerman, Thimo Kurz, Lionel Pintard, Pierre Gönczy, Danielle R. Hamill
Rok vydání: 2002
Předmět:
Cell Nucleus/physiology/ultrastructure
Embryo
Nonmammalian

Microtubules/drug effects/*physiology/ultrastructure
Ubiquitins/genetics/*metabolism
Cell Membrane/ultrastructure
Cell Cycle Proteins
P.H.S
Microtubules
NEDD8
Pronuclear migration
Non-U.S. Gov't
Genes
Helminth

Adenosine Triphosphatases
Multidisciplinary
Nocodazole
Helminth/genetics
Cullin Proteins
Nocodazole/pharmacology
Cell biology
Ubiquitin ligase
Actin Cytoskeleton
Meiosis
Embryo
Mitotic Spindle Apparatus/physiology/ultrastructure
Katanin
Caenorhabditis elegans/*embryology/genetics/metabolism
Cell Division
Cullin
Caenorhabditis elegans Proteins/genetics/*metabolism
Mitosis
Spindle Apparatus
Biology
Research Support
Nonmammalian/metabolism
Helminth
Animals
Caenorhabditis elegans
Caenorhabditis elegans Proteins
Ubiquitins
Cell Nucleus
Cell Membrane
Spindle apparatus
Genes
Adenosinetriphosphatase/genetics/*metabolism
Mutation
biology.protein
Microfilaments/*physiology/ultrastructure
RNA
U.S. Gov't
RNA
Helminth

Cell Cycle Proteins/genetics/metabolism
Cytokinesis
Zdroj: Science. 295:1294-1298
ISSN: 1095-9203
0036-8075
DOI: 10.1126/science.1067765
Popis: The Nedd8 ubiquitin-like protein modification pathway regulates cell-cycle progression. Our analysis of Nedd8 requirements during Caenorhabditis elegans embryogenesis indicates that the cytoskeleton is another target. Nedd8 conjugation negatively regulated contractility of the microfilament-rich cell cortex during pronuclear migration and again during cytokinesis. The Nedd8 pathway also was required after meiosis to negatively regulate katanin, a microtubule-severing complex, permitting the assembly of a large mitotic spindle. We propose that Nedd8-modified cullin, as part of an E3 ubiquitin ligase complex, targets katanin for degradation during the transition from meiosis to mitosis.
Databáze: OpenAIRE
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