Development of an immunochromatographic test for the detection of Mycoplasma pneumoniae GroES antigen
| Autor: | Makoto Sugimura, Megumi Inoue-Tsuda, Hidehito Matsui, Hideaki Hanaki, Kazuya Iwabuchi |
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| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Mycoplasma pneumoniae medicine.drug_class Antibiotics Microbial Sensitivity Tests Cross Reactions Monoclonal antibody medicine.disease_cause Microbiology Chromatography Affinity law.invention Mice Antigen law Cell Wall Pneumonia Mycoplasma medicine Chaperonin 10 Animals Molecular Biology Antigens Bacterial Hybridomas biology Diagnostic Tests Routine Antibodies Monoclonal GroES biology.organism_classification Anti-Bacterial Agents Community-Acquired Infections Recombinant DNA biology.protein Macrolides Antibody Bacteria |
| Zdroj: | Journal of microbiological methods. 191 |
| ISSN: | 1872-8359 |
| Popis: | Mycoplasma pneumoniae frequently causes community-acquired pneumonia in children; β-lactam antibiotics are ineffective against this bacterium because of its lack of a cell wall. Hence, a rapid and simple detection method is required to ensure appropriate treatment. In this study, we developed a rapid and simple immunochromatography-based detection method using monoclonal antibodies that react with the co-chaperone GroES of M. pneumoniae . Mice were immunized with recombinant GroES, and hybridoma cells producing anti-GroES monoclonal antibodies were established. For the development of the immunochromatographic test, antibody pairs with superior reactivity and specificity were selected. The developed immunochromatographic test could detect 0.1 ng/mL of recombinant GroES within 20 min. Moreover, no cross-reaction was observed with other microorganisms, including six Mycoplasma species, 20 other bacterial species, and one yeast species. Macrolide-resistant and -susceptible M. pneumoniae clinical isolates were detected at approximately 104 to 105 colony-forming units/mL. The study indicates that immunochromatographic tests targeting GroES are useful for rapid and simple detection of M. pneumoniae. |
| Databáze: | OpenAIRE |
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