Artemisinin Activity in Red Blood Cells from Anemic Children
| Autor: | Fatou Joof, Morgan M. Goheen, Carla Cerami |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Drug
Male Erythrocytes Anemia media_common.quotation_subject Plasmodium falciparum Drug Resistance Pharmacology chemistry.chemical_compound Antimalarials Gentamicin protection assay Virology parasitic diseases medicine Humans Artemisinin Heme media_common chemistry.chemical_classification Reactive oxygen species biology Anemia Iron-Deficiency business.industry Infant Articles medicine.disease biology.organism_classification Artemisinins Infectious Diseases chemistry Parasitology Female Gambia business Malaria medicine.drug |
| Zdroj: | Am J Trop Med Hyg |
| ISSN: | 1476-1645 0002-9637 |
| Popis: | Artemisinin combination therapies are the current frontline therapy for falciparum malaria. Artemisinin is activated by heme iron, and the consequent production of reactive oxygen species and carbon-centered radicals results in rapid parasite clearance. Red blood cells (RBCs) from anemic iron-deficient individuals have decreased levels of heme, and such deficiencies are highly prevalent among children and pregnant women in malaria-endemic countries. We, therefore, investigated the possibility that host anemia could impair artemisinin activity and alter the drug sensitivity of artemisinin-resistant strains of Plasmodium falciparum. We collected RBCs from anemic (n = 35) and nonanemic (n = 11) Gambian children between the ages of 2 and 24 months. Parasites grown in RBCs from both groups were assessed in vitro using the ring-stage survival assay with artemisinin-resistant and artemisinin-sensitive strains of P. falciparum. No differences were found in artemisinin sensitivity (P > 0.05), and there was no correlation between artemisinin activity and host hemoglobin levels. Standard antimalarial drug activity assays for representatives of the major classes of antimalarial drugs found no differences in the IC(50) values against P. falciparum between anemic and nonanemic RBCs. We conclude that host anemia does not influence artemisinin activity. |
| Databáze: | OpenAIRE |
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