Glucose Signaling Is Important for Nutrient Adaptation during Differentiation of Pleomorphic African Trypanosomes
| Autor: | Jessica A. Jones, Rooksana E. Noorai, Yijian Qiu, Jillian E. Milanes, Vijay Shankar, James Morris |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Molecular Biology and Physiology Trypanosoma RNA splicing carbon metabolism 030106 microbiology Trypanosoma brucei brucei translation Trypanosoma brucei Biology Microbiology 03 medical and health sciences Downregulation and upregulation Stress Physiological Gene expression Parasite hosting Hexose Molecular Biology 030304 developmental biology chemistry.chemical_classification 0303 health sciences Life Cycle Stages 030306 microbiology Cell growth glucose sensing fungi Primary metabolite biology.organism_classification Adaptation Physiological QR1-502 Amino acid Cell biology Metabolic pathway 030104 developmental biology Glucose chemistry mRNA degradation Commentary Adaptation Energy source Energy Metabolism Signal Transduction |
| Zdroj: | mSphere, Vol 3, Iss 5 (2018) mSphere |
| ISSN: | 2379-5042 |
| Popis: | Salivarian trypanosomes grow in mammals, where they depend on glucose, and as procyclic forms in tsetse flies, where they metabolize proline. Differentiation of bloodstream forms to nongrowing stumpy forms, and to procyclic forms, has been studied extensively, but reconciling the results is tricky because investigators have used parasites with various differentiation competences and different media for procyclic-form culture. Salivarian trypanosomes grow in mammals, where they depend on glucose, and as procyclic forms in tsetse flies, where they metabolize proline. Differentiation of bloodstream forms to nongrowing stumpy forms, and to procyclic forms, has been studied extensively, but reconciling the results is tricky because investigators have used parasites with various differentiation competences and different media for procyclic-form culture. Standard protocols include lowering the temperature to 27°C, adding a tricarboxylic acid, and transferring the parasites to high-proline medium, often including glucose. A 20°C cold shock enhanced efficiency. Y. Qiu, J. E. Milanes, J. A. Jones, R. E. Noorai, et al. (mSphere 3:e00366-18, 2018, https://doi.org/10.1128/mSphere.00366-18) studied this systematically, and their results call long-established protocols into question. Importantly, highly efficient differentiation was observed after cold shock and transfer to no-glucose medium without tricarboxylic acid; in contrast, glucose made differentiation tricarboxylic acid dependent and inhibited procyclic growth. New transcriptome data for stumpy and procyclic forms will enable informative comparisons with biochemical observations and with other RNA and protein data sets. |
| Databáze: | OpenAIRE |
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