Docosahexaenoic acid enhances the efficacy of docetaxel in prostate cancer cells by modulation of apoptosis: The role of genes associated with the NF-κB pathway
| Autor: | Iain Brown, Irshad A.A. Shaikh, Klaus W. J. Wahle, Andrew Craig Schofield, Steven D. Heys |
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| Rok vydání: | 2008 |
| Předmět: |
Male
medicine.medical_specialty Docosahexaenoic Acids Urology Gene Expression Antineoplastic Agents Apoptosis Docetaxel urologic and male genital diseases Prostate cancer DU145 Cell Line Tumor Internal medicine LNCaP medicine Humans Cytotoxic T cell Viability assay Membrane Potential Mitochondrial Reverse Transcriptase Polymerase Chain Reaction business.industry Cell Cycle NF-kappa B Prostatic Neoplasms Reproducibility of Results Cancer Drug Synergism medicine.disease Endocrinology Oncology Cancer research Taxoids business Signal Transduction medicine.drug |
| Zdroj: | The Prostate. 68:1635-1646 |
| ISSN: | 1097-0045 0270-4137 |
| Popis: | BACKGROUND Evidence is growing for beneficial interactions between omega-3 fatty acids from fish and chemotherapy agents in certain human cancers. Evidence for similar effects in prostate cancer is lacking. We investigated the effects of docosahexaenoic acid (DHA-22:6n-3), a component of fish oil, on the cytotoxicity of docetaxel in prostate cancer cells. METHODS Cell viability was studied using the MTT assay and apoptosis was evaluated by flow cytometry using PI, annexin V, and JC-1 staining. Cellular signaling mechanisms that might explain the observed pro-apoptotic effects were investigated using NF-κB pathway specific cDNA microarrays and RT-PCR validation. RESULTS DHA enhanced the pro-apoptotic efficacy of docetaxel, synergistically, in hormone receptor positive and negative LNCaP, DU145 and PC3 cells, respectively. Cell cycle analysis showed an increase in G2M arrest and JC-1 staining showed a significant (P |
| Databáze: | OpenAIRE |
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