Congenital toxoplasmosis: Serology, PCR, parasite isolation and molecular characterization of Toxoplasma gondii

Autor: Ricardo Durlach, Liliana Carral, Gastón Moré, Cristina Freuler, María Cecilia Venturini, Federico Kaufer, Lais Luján Pardini
Jazyk: Spanish; Castilian
Rok vydání: 2018
Předmět:
0301 basic medicine
medicine.medical_specialty
CIENCIAS MÉDICAS Y DE LA SALUD
030231 tropical medicine
Ciencias de la Salud
Immunoglobulin E
Serology
purl.org/becyt/ford/3.3 [https]
03 medical and health sciences
0302 clinical medicine
Epidemiology
Genotype
Medicine
Parasite hosting
Parasitología
MOLECULAR CHARACTERIZATION
serología
Pregnancy
Congenital toxoplasmosis
biology
Toxoplasmosis Congénita
business.industry
Ciencias Veterinarias
SEROLOGY
Public Health
Environmental and Occupational Health
caracterización molecular
Parasitic isolation
030108 mycology & parasitology
medicine.disease
Aislamiento del parásito
Toxoplasmosis
Molecular characterization
Serología
Caracterización molecular
Infectious Diseases
PCR
PARASITIC ISOLATION
Toxoplasmosis congénita
Immunology
biology.protein
RPC
purl.org/becyt/ford/3 [https]
aislamiento del parásito
Antibody
business
CONGENITAL TOXOPLASMOSIS
Zdroj: CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
Revista chilena de infectología v.35 n.1 2018
SciELO Chile
CONICYT Chile
instacron:CONICYT
SEDICI (UNLP)
Universidad Nacional de La Plata
instacron:UNLP
Popis: Background. Congenital toxoplasmosis diagnosis in the newborn is a very important issue due to the need for early treatment to prevent future sequels. Aim: To compare available methods at the institution for the diagnosis of congenital toxoplasmosis. Material and Methods: In this study we have evaluated the different diagnostic tests used in 67 congenital exposed newborns, including serological tests, PCR, parasite isolation and molecular characterization. Results: The ISAGA IgM and IgA tests showed sensitivity (Se) of 87 and 91%, respectively, and specificity (Sp) of 100%. When ISAGA IgM and IgA were performed simultaneously, the Se increased to 98% and the Sp was 100%. The presence of IgE contributed to the diagnosis when it was detected in the child’s serum but not in maternal blood. In four congenital infected children the parasite was isolated and genotyped: one was genotype II and the other three were “atypical” genotypes. No parasite was isolated in children without congenital toxoplasmosis. Discussion: Overall, serological tests showed a good diagnostic performance although in one case they were all negative and isolation was the only tool to identify the infection. We conclude that it is essential to use all diagnostic tests in every single exposed child, including if possible, molecular characterization due to its epidemiological implication.
Facultad de Ciencias Veterinarias
Databáze: OpenAIRE
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