Effect of co-axially hybridized gene targets on hybridization efficiency of microarrayed DNA probes
| Autor: | Hung-Ju Su, Kai Ren Jiang, Jui-Chuang Wu, Chia-Chun Chen, Jie-Len Huang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Molecular steric hindrance
DNA pre-hybridization DNA biochips Gene targets General Chemical Engineering Hybridization probe DNA microarray General Chemistry Biology Fluorescence Molecular biology Article chemistry.chemical_compound chemistry Hybridization efficiency Biophysics Multiplex ligation-dependent probe amplification Axial symmetry DNA |
| Zdroj: | Journal of the Taiwan Institute of Chemical Engineers |
| ISSN: | 1876-1089 1876-1070 |
| Popis: | The effect of relative size of two co-axially hybridized gene targets on the hybridization efficiency was studied for two DNA probe configurations and various probe concentrations. Each of two sets of microarrayed probes contained a pair of DNA probes and a pair of their complementary samples labeled with two distinct fluorescent dyes. The sequence of each probe is especially designed so that two targets are simultaneously complementary to two adjacent sections of the probe. The molecular steric effect on the hybridization efficiency is investigated by comparing the dye signals between configurations of one-target and two-target hybridization scenarios. The results show that a low probe concentration gives better hybridization efficiency and the first-hybridization conducted by a shorter-size DNA target improves the hybridization efficiency of the second target coupling onto the same probe. |
| Databáze: | OpenAIRE |
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