Differential Diagnostics of Active Progressing Multiple Sclerosis Using a Fluorescent Biomarker with Resonance Energy Transfer
| Autor: | N. N. Kostin, A. A. Kudryaeva, S. S. Terekhov, M. N. Zakharova, Alexander G. Gabibov, Alexey A. Belogurov, M. V. Ivanova, Ya. A. Lomakin, Alena N. Kaminskaya, T. O. Simaniv |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Male 0301 basic medicine Multiple Sclerosis Fluorophore General Biochemistry Genetics and Molecular Biology Diagnosis Differential Young Adult 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Humans Aged Autoantibodies Fluorescent Dyes biology Chemistry Multiple sclerosis Experimental autoimmune encephalomyelitis Autoantibody Myelin Basic Protein General Medicine Carbocyanines Middle Aged medicine.disease Fluorescence Myelin basic protein 030104 developmental biology Energy Transfer Biochemistry biology.protein Biomarker (medicine) Female Antibody Biomarkers 030217 neurology & neurosurgery |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 167:329-334 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/s10517-019-04520-1 |
| Popis: | Previous data showed that myelin-reactive autoantibodies found in patients with multiple sclerosis and mice with experimental autoimmune encephalomyelitis recognize and hydrolyze various fragments of myelin basic protein (MBP). Moreover, antibody-mediated cleavage of the encephalithogenic fragment MBP81-103 flanked with two fluorescent proteins can serve as a new biomarker of multiple sclerosis. Here we describe creation of the next generation of this biomarker based on antibody-dependent degradation of a new chemically synthesized fluorescent substrate with resonance energy transfer that contains fluorophore Cy5 and quencher QXL680 separated by MBP81-99 protein (Cy5-MBP81-99-QXL680). This substrate is degraded during incubation with purified antibodies and B cells from patients with multiple sclerosis, but not healthy volunteers. |
| Databáze: | OpenAIRE |
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