Kribbella aluminosa sp. nov., isolated from a medieval alum slate mine
| Autor: | Marc René Carlsohn, Erko Stackebrandt, Ingrid Groth, Thomas Munder, Hans-Peter Saluz, Barbara Schütze, Cathrin Spröer |
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| Rok vydání: | 2007 |
| Předmět: |
DNA
Bacterial Kribbella aluminosa Molecular Sequence Data Carbohydrates Mannose Peptidoglycan Biology Diaminopimelic Acid Microbiology DNA Ribosomal Mining chemistry.chemical_compound Germany RNA Ribosomal 16S Sequence Homology Nucleic Acid Botany Actinomycetales Cluster Analysis Ecology Evolution Behavior and Systematics Phospholipids Phylogeny Soil Microbiology chemistry.chemical_classification Phylogenetic tree Alum Fatty Acids Fatty acid Nucleic Acid Hybridization Genes rRNA General Medicine Sequence Analysis DNA 16S ribosomal RNA DNA Fingerprinting Bacterial Typing Techniques RNA Bacterial Kribbella solani chemistry Alum Compounds Glycolipids |
| Zdroj: | International journal of systematic and evolutionary microbiology. 57(Pt 9) |
| ISSN: | 1466-5026 |
| Popis: | Three actinomycetes (strains HKI 0478T, HKI 0479 and HKI 0480) isolated from the surfaces of rocks in the Feengrotten medieval alum slate mine (Thuringia, Germany) were examined in a polyphasic taxonomic study. The following morphological and chemotaxonomic features supported their classification as members of the genus Kribbella: the presence of ll-diaminopimelic acid in the cell-wall peptidoglycan; glucose together with minor amounts of mannose and ribose as the whole-cell sugars; polar lipids comprising phosphatidylcholine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and unknown phospho- and glycolipids; fatty acid profiles characterized by the predominance of anteiso-C15 : 0, iso-C16 : 0 and C16 : 0 9-methyl; and the presence of MK-9(H4) as the main menaquinone. The isolates had almost identical 16S rRNA gene sequences (99.9–100 %) and were most closely related to the type strains of Kribbella jejuensis (98.9 % sequence similarity), Kribbella swartbergensis and Kribbella solani (both 98.8 %). A wide range of genotypic and phenotypic markers as well as the low levels of DNA–DNA relatedness between strain HKI 0478T and the type strains of K. jejuensis (41.3 %), K. swartbergensis (18.6 %) and K. solani (14.2 %) distinguished the novel strains from their closest phylogenetic neighbours. On the basis of these results, strain HKI 0478T represents a novel member of the genus Kribbella, for which the name Kribbella aluminosa sp. nov. is proposed. The type strain is HKI 0478T (=DSM 18824T =JCM 14599T). |
| Databáze: | OpenAIRE |
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