A New and Frequent Human T-Cell Leukemia Virus Indeterminate Western Blot Pattern: Epidemiological Determinants and PCR Results in Central African Inhabitants
| Autor: | Patricia Tortevoye, Micheline Guillotte, Sara Calattini, Odile Mercereau-Puijalon, Sabine Plancoulaine, Sylviane Bassot, Claudia Filippone, Antoine Gessain, Edouard Betsem |
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| Přispěvatelé: | Hôpital de l'Institut Pasteur [Paris], Institut Pasteur [Paris], Epidémiologie et Physiopathologie des Virus Oncogènes, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Université de Yaoundé I, Immunologie moléculaire des parasites, Institut National de la Santé et de la Recherche Médicale (INSERM), This work was supported by the Institut Pasteur and the Association Virus Cancer Prevention. We acknowledge the region Ile de France (postdoctoral grant DIM-Malinf) and the European program EMPERIE for financial support to Claudia Filippone., we thank the Service de Coopération et de l'Action Culturelle (SCAC) of the French embassy in Yaounde and the Institut National pour le Cancer for supporting Edouard Betsem., European Project: 223498,EC:FP7:HEALTH,FP7-HEALTH-2007-B,EMPERIE(2009) |
| Rok vydání: | 2012 |
| Předmět: |
Male
Epidemiology viruses Antibodies Viral Polymerase Chain Reaction law.invention Serology MESH: Aged 80 and over Seroepidemiologic Studies law MESH: Child Ethnicity Child ComputingMilieux_MISCELLANEOUS Polymerase chain reaction MESH: Aged Aged 80 and over Human T-lymphotropic virus 1 0303 health sciences MESH: Middle Aged biology Human T-lymphotropic virus 2 virus diseases Middle Aged Blot [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology MESH: Young Adult Child Preschool Female Indeterminate MESH: Leukemia T-Cell Adult Microbiology (medical) Leukemia T-Cell Adolescent Blotting Western Virus Young Adult 03 medical and health sciences MESH: Ethnicity MESH: Blotting Western Humans Seroprevalence Africa Central Aged 030304 developmental biology MESH: Adolescent MESH: Human T-lymphotropic virus 1 MESH: Humans MESH: Seroepidemiologic Studies MESH: Human T-lymphotropic virus 2 030306 microbiology MESH: Child Preschool MESH: Adult MESH: Polymerase Chain Reaction biology.organism_classification Virology MESH: Male MESH: Africa Central Immunology MESH: Female MESH: Antibodies Viral |
| Zdroj: | Journal of Clinical Microbiology Journal of Clinical Microbiology, American Society for Microbiology, 2012, 50 (5), pp.1663-1672. ⟨10.1128/JCM.06540-11⟩ Journal of Clinical Microbiology, 2012, 50 (5), pp.1663-1672. ⟨10.1128/JCM.06540-11⟩ |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.06540-11 |
| Popis: | Human T-cell leukemia virus (HTLV) indeterminate Western blot (WB) serological patterns are frequently observed in plasma/serum from persons living in intertropical areas. In the framework of ongoing projects on HTLV-1/2 and related viruses in Central Africa, we systematically analyzed plasma from villagers living in South Cameroon by WB. The group included 1,968 individuals (mean age, 44 years; age range, 5 to 90 years; 978 women/990 men), both Bantus (1,165) and Pygmies (803). Plasma samples were tested by WB analysis (MPD HTLV Blot 2.4) and interpreted according to the manufacturer's instructions. Only clear bands were considered in the analysis. Among the 1,968 plasma samples, 38 (1.93%) were HTLV-1, 13 (0.66%) were HTLV-2, and 6 (0.3%) were HTLV WB seropositive. Furthermore, 1,292 (65.65%) samples were WB sero-indeterminate, including 104 (5.28%) with an HTLV-1 Gag-indeterminate pattern (HGIP) and 68 (3.45%) with a peculiar yet unreported pattern exhibiting mostly a strong shifted GD21 and a p28. The other 619 (31.45%) samples were either WB negative or exhibited other patterns, mostly with unique p19 or p24 bands. DNA, extracted from peripheral blood buffy coat, was subjected to PCR using several primer pairs known to detect HTLV-1/2/3/4. Most DNAs from HTLV-1- and HTLV-seropositive individuals were PCR positive. In contrast, all the others, from persons with HTLV-2, HGIP, new WB, and other indeterminate patterns, were PCR negative. Epidemiological determinant analysis of the persons with this new peculiar WB pattern revealed that seroprevalence was independent from age, sex, or ethnicity, thus resembling the indeterminate profile HGIP rather than HTLV-1. Moreover, this new pattern persists over time. |
| Databáze: | OpenAIRE |
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