MicroRNA profiling of the intestinal tissue of Kazakh sheep after experimental Echinococcus granulosus infection, using a high-throughput approach
| Autor: | Xin Li, Xuhai Wang, Qian Ban, Song Jiang, Wenqiao Hui, Bin Jia |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Sequence analysis Veterinary (miscellaneous) 030231 tropical medicine Sheep Diseases Kazakh Major histocompatibility complex MHC haplotype lcsh:Infectious and parasitic diseases Major Histocompatibility Complex 03 medical and health sciences 0302 clinical medicine Echinococcosis microRNA Gene expression medicine Cystic Echinococcosis Animals lcsh:RC109-216 Echinococcus granulosus Disease Resistance Genetics Sheep biology Sequence Analysis RNA Haplotype Zoonosis High-Throughput Nucleotide Sequencing medicine.disease biology.organism_classification language.human_language Echinococcus Intestines MicroRNAs 030104 developmental biology Infectious Diseases Haplotypes Insect Science biology.protein language Animal Science and Zoology Parasitology Female Research Article |
| Zdroj: | Parasite Parasite, Vol 23, p 23 (2016) |
| ISSN: | 1776-1042 1252-607X |
| Popis: | Cystic echinococcosis (CE), caused by infection with the larval stage of the cestode Echinococcus granulosus , is a chronic zoonosis, to which sheep are highly susceptible. Previously, we found that Kazakh sheep with different MHC haplotypes differed in CE infection. Sheep with haplotype MHCMva Ibc-Sac IIab-Hin 1Iab were resistant to CE infection, while their counterparts without this haplotype were not. MicroRNAs (miRNAs), a class of small non-coding RNAs, are key regulators of gene expression at the post-transcriptional level and play essential roles in fundamental biological processes such as development and metabolism. To identify microRNA controlling resistance to CE in the early stage of infection, microRNA profiling was conducted in the intestinal tissue of sheep with resistant and non-resistant MHC haplotypes after peroral infection with E. granulosus eggs. A total of 351 known and 186 novel miRNAs were detected in the resistant group, against 353 known and 129 novel miRNAs in the non-resistant group. Among these miRNAs, 83 known miRNAs were significantly differentially expressed, including 75 up-regulated and 8 down-regulated miRNAs. Among these known microRNAs, miR-21-3p, miR-542-5p, miR-671, miR-134-5p, miR-26b, and miR-27a showed a significantly higher expression in CE-resistant sheep compared to the CE-non-resistant library, with the FC > 3. Functional analysis showed that they were NF-kB pathway-responsive miRNAs, which are involved in the inflammation process. The results suggest that these microRNAs may play important roles in the response of intestinal tissue to E. granulosus . |
| Databáze: | OpenAIRE |
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