Molecular cloning of kisspeptin receptor genes (gpr54-1 and gpr54-2) and their expression profiles in the brain of a tropical damselfish during different gonadal stages
| Autor: | Sung Pyo Hur, Yuki Takeuchi, Akihiro Takemura, Selma Bouchekioua, Satoshi Imamura |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty DNA Complementary Physiology 030209 endocrinology & metabolism Receptors Cell Surface Biology Biochemistry 03 medical and health sciences Diencephalon 0302 clinical medicine Kisspeptin Complementary DNA Internal medicine medicine Animals Amino Acid Sequence RNA Messenger Receptor Molecular Biology Phylogeny Messenger RNA Kisspeptins Base Sequence Chrysiptera cyanea Gene Expression Profiling Fishes Brain biology.organism_classification Cell biology Gene expression profiling 030104 developmental biology Endocrinology Female Vitellogenesis hormones hormone substitutes and hormone antagonists |
| Zdroj: | Comparative biochemistry and physiology. Part A, Molecularintegrative physiology. 203 |
| ISSN: | 1531-4332 |
| Popis: | The kisspeptin receptor (GPR54) mediates neuroendocrine control of kisspeptin in the brain and acts as a gateway for a pulsatile release of hypothalamic gonadotropin-releasing hormone. This study aimed to clone two GPR54 genes (gpr54-1 and gpr54-2) from the brain of the sapphire devil Chrysiptera cyanea, a tropical damselfish, and to study their involvement in reproduction. The partial sequences of the sapphire devil gpr54-1 cDNA (1059bp) and gpr54-2 cDNA (1098bp) each had an open reading frame encoding a protein of 353 and 366 amino acids, respectively, both of which had structural features of a G-protein-coupled receptor. The expression of gpr54-1 mRNA was observed in the diencephalon and telencephalon, and gpr54-2 mRNA was found in the optic tectum of sapphire devil. When gpr54-1 and gpr54-2 mRNA levels were examined in the brain of sapphire devil by real-time quantitative polymerase chain reaction (qPCR), they were found to increase during late vitellogenesis and post-spawning. Treatment of fish with estradiol-17β (E2) resulted in an increase in gpr54-1 and gpr54-2 expression in the brain of sapphire devil. Thus, kisspeptin receptors likely mediate the activity of kisspeptin in the brain and are involved in controlling reproductive events in a tropical damselfish. |
| Databáze: | OpenAIRE |
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