Valproic Acid Estimation by Enzyme Immunoassay
| Autor: | P. T. Lascelles, Neville Ratnaraj, A.A. Elyas, Valerie D. Goldberg |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
030213 general clinical medicine
Chromatography Gas Sodium Clinical Biochemistry chemistry.chemical_element 030209 endocrinology & metabolism Oxalate Specimen Handling Immunoenzyme Techniques Fluorides 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Therapeutic index medicine Humans False Positive Reactions Edetic Acid chemistry.chemical_classification Oxalates Valproic Acid Chromatography medicine.diagnostic_test General Medicine Enzyme chemistry Reagent Immunoassay lipids (amino acids peptides and proteins) Reagent Kits Diagnostic Fluoride medicine.drug |
| Zdroj: | Annals of Clinical Biochemistry: International Journal of Laboratory Medicine. 17:307-310 |
| ISSN: | 1758-1001 0004-5632 |
| DOI: | 10.1177/000456328001700605 |
| Popis: | Blood samples from patients on long-term sodium valproate (Epilim) therapy were analysed for valproic acid by two procedures, enzyme immunoassay (EMIT)* and gas-liquid chromatography (GLC). A critical evaluation of the effects of anticoagulants added to specimens was performed in addition to studies on reagent specificity, accuracy, and precision. EMIT valproic acid reagent showed high specificity, and there was a good correlation between results obtained by EMIT and GLC over a wide range of concentrations. The accuracy and precision of EMIT assay was good over the therapeutic range of valproic acid. The presence of the anticoagulant EDTA or of fluoride/oxalate produced a bias towards high results for valproic acid as compared with those obtained from the analysis of serum. |
| Databáze: | OpenAIRE |
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