Effect of specimen storage conditions on newborn dried blood spots used to assess Toxoplasma gondii immunoglobulin M (IgM)
| Autor: | Margaret A. Honein, W. Harry Hannon, Jeffrey L. Jones, Sonja A. Rasmussen, Shu Chaing, Joanne V. Mei, Lixia Li, Fred Lorey, Gary M. Shaw, Mark A. Canfield, Sarah A. Collier, Jaime L. Frias, Robert E. Meyer |
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| Rok vydání: | 2011 |
| Předmět: |
Clinical Biochemistry
Population Physiology Biology Sensitivity and Specificity Biochemistry Congenital hydrocephalus Animals Humans Dried blood education Blood Specimen Collection Newborn screening education.field_of_study Spots Biochemistry (medical) Infant Newborn Toxoplasma gondii General Medicine biology.organism_classification nervous system diseases Immunoglobulin M Immunology biology.protein Toxoplasma Blood Chemical Analysis Hydrocephalus |
| Zdroj: | Clinica Chimica Acta. 412:455-459 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/j.cca.2010.11.028 |
| Popis: | Background Newborn screening programs store—under varying conditions—residual dried blood spots (DBS). Residual DBS were used to investigate the contribution of congenital infection with Toxoplasma gondii to the etiology of hydrocephalus and as a key step, we assessed the effect of storage conditions on the stability of newborn screening biomarkers. Methods Infants with hydrocephalus (410 cases) were identified using population-based birth defects surveillance systems in California, North Carolina, and Texas. Infants without birth defects (448 controls) were randomly selected from the same geographic areas and time periods. California stores DBS with controlled temperature, while North Carolina and Texas store DBS under ambient conditions. After removal of personal identifiers, DBS were tested for Toxo-specific immunoglobulin-M (Toxo-IgM). Because of poor elution of DBS stored in ambient conditions, additional biomarkers were tested on a specimen subset. Results Among 858 DBS tested, Toxo-IgM was found in 3 cases and no controls from California ( N = 515) and in no specimens from North Carolina or Texas ( N = 343). Among the 98 specimens tested for selected biomarkers, statistically significant differences were found for California vs. combined North Carolina and Texas DBS (thyroid stimulating hormone, phenylalanine, methionine, leucine and citrulline p p Conclusions Storage conditions for residual DBS had an effect on the ability to extract, recover, and accurately measure Toxo-IgM and other biomarkers from the filter paper matrix. |
| Databáze: | OpenAIRE |
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