The amoebicidal activity of three substances derived from benzothiazole on Acanthamoeba castellanii cysts and trophozoites and its cytotoxic potentials
| Autor: | Tuğba Kaya, Hasan Yucel, Necati Ozpinar, Gülnaz Çulha |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Veterinary (miscellaneous) 030231 tropical medicine Keratitis Microbiology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine parasitic diseases medicine Parasite hosting Cyst Amebicides Benzothiazoles Trophozoites Axenic Air filter Acanthamoeba castellanii biology Amebiasis 030108 mycology & parasitology medicine.disease biology.organism_classification Acanthamoeba Infectious Diseases Benzothiazole chemistry Insect Science Parasitology |
| Zdroj: | Acta Tropica. 220:105981 |
| ISSN: | 0001-706X |
| Popis: | Acanthamoeba species are free-living amoebae isolated from many ecological areas such as swimming pools, dams, lakes, soil, and air filters. These amoebae are usually causing granulomatous amebic encephalitis and amebic keratitis in immunosuppressive individuals. In this study, the reproductive potential and morphological changes determined of Acanthamoeba castellanii trophozoite and cyst forms exposed to three different active substances derived from benzothiazole. Furthermore, the cytotoxic potential of these active substances determined by XTT analysis. In the study, axenic cultures prepared for Acanthamoeba castellanii cyst and trophozoite forms and parasite exposed to different concentrations of active substances. Cell counts of parasite cultures were performed at the 30 minutes, 1st, 6th, 12th, 24th, and 48th hour periods. As a result of the study, the reproductive potential suppressive effects of all three substances on Acanthamoeba castellanii trophozoites and cysts were determined. The most effective of these substances was 2-Amino-6(trifluoromethoxy)-benzothiazole. In the first three concentrations of this substance (0.1%, 0.05%, 0.025%), no determined trophozoite and cysts at the end of twenty four. Due to its strong ameobicidal effect, it is thought that 2-Amino-6(trifluoromethoxy)-benzothiazole may be a new therapeutic agent in diseases caused by acanthamoeba parasites by supporting this study with animal experiments. |
| Databáze: | OpenAIRE |
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